Reference - Detail
RRC ID | 71452 |
---|---|
Author | Sagara Y, Hara T, Ariyasu Y, Ando F, Tokunaga N, Horiuchi T. |
Title | Direct expression in Escherichia coli and characterization of bovine adrenodoxins with modified amino-terminal regions. |
Journal | FEBS Lett |
Abstract |
Four forms of bovine adrenodoxin with modified amino-termini obtained by direct expression of cDNAs in Escherichia coli are Ad(Met1), Ad(Met-1), Ad(Met-12), and Ad(Met6). The shoulder numbers represent the site of translation initiator Met at the amino-termini. The adrenodoxins, except for Ad(Met-1), were purified from the cell lysate and the ratios of A414-to-A276 of the purified proteins were over 0.92. NADPH-cytochrome c reductase activities of the three forms of adrenodoxin in the presence of adrenodoxin reductase were the same as that of purified bovine adrenocortical adrenodoxin. However, as cytochrome P-450SCC reduction catalyzed by Ad(Met6) was about 60% of that by Ad(Met1), the contribution of the amino-terminal region for the electron transfer or binding to cytochrome P-450SCC would need to be considered. |
Volume | 300(3) |
Pages | 208-12 |
Published | 1992-4-6 |
DOI | 10.1016/0014-5793(92)80847-a |
PII | 0014-5793(92)80847-A |
PMID | 1555645 |
MeSH | Adrenodoxin / chemistry Adrenodoxin / genetics* Adrenodoxin / isolation & purification Amino Acid Sequence Animals Base Sequence Catalysis Cattle Cloning, Molecular Escherichia coli / genetics* Genetic Vectors Molecular Sequence Data Recombinant Proteins / chemistry* Recombinant Proteins / isolation & purification Structure-Activity Relationship |
IF | 3.057 |
Resource | |
DNA material | pBA1159 (RDB19560) |