| RRC ID |
71471
|
| 著者 |
Oguchi M, Jiasen J, Yoshioka TW, Tanaka YR, Inoue K, Takada M, Kikusui T, Nomoto K, Sakagami M.
|
| タイトル |
Microendoscopic calcium imaging of the primary visual cortex of behaving macaques.
|
| ジャーナル |
Sci Rep
|
| Abstract |
In vivo calcium imaging with genetically encoded indicators has recently been applied to macaque brains to monitor neural activities from a large population of cells simultaneously. Microendoscopic calcium imaging combined with implantable gradient index lenses captures neural activities from deep brain areas with a compact and convenient setup; however, this has been limited to rodents and marmosets. Here, we developed miniature fluorescent microscopy to image neural activities from the primary visual cortex of behaving macaques. We found tens of clear fluorescent signals from three of the six brain hemispheres. A subset of these neurons showed clear retinotopy and orientation tuning. Moreover, we successfully decoded the stimulus orientation and tracked the cells across days. These results indicate that microendoscopic calcium imaging is feasible and reasonable for investigating neural circuits in the macaque brain by monitoring fluorescent signals from a large number of neurons.
|
| 巻・号 |
11(1)
|
| ページ |
17021
|
| 公開日 |
2021-8-23
|
| DOI |
10.1038/s41598-021-96532-z
|
| PII |
10.1038/s41598-021-96532-z
|
| PMID |
34426639
|
| PMC |
PMC8382832
|
| MeSH |
Animals
Behavior, Animal / physiology*
Calcium / metabolism*
Endoscopy*
Fixation, Ocular / physiology
Fluorescence
Genetic Vectors / administration & dosage
Imaging, Three-Dimensional*
Injections
Lenses, Intraocular
Macaca
Male
Neurons / physiology
Orientation
Photic Stimulation
Visual Cortex / diagnostic imaging*
Visual Cortex / virology
Visual Fields / physiology
|
| IF |
3.998
|
| リソース情報 |
| ニホンザル |
|
