Reference - Detail
| RRC ID | 7150 |
|---|---|
| Author | Hirose Y, Murosaki S, Fujiki T, Yamamoto Y, Yoshikai Y, Yamashita M. |
| Title | Lipoteichoic acids on Lactobacillus plantarum cell surfaces correlate with induction of interleukin-12p40 production. |
| Journal | Microbiol Immunol |
| Abstract |
Heat-killed cells of Lactobacillus plantarum L-137 are potent inducers of IL-12 in vitro as well as in vivo and have been shown to have antiallergic, antitumor, and antiviral effects through this induction, which leads to a Th1 type immune response. To determine why L-137 cells induce much greater IL-12 production than the type strain Lactobacillus plantarum JCM1149, we examined the differences in their CW components. The L-137 CW was found to have a higher alanine content and IL-12p40 induction was significantly greater in comparison with JCM1149 CW, whereas peptidoglycans isolated from both strains did not cause IL-12p40 induction. Because in purified CW preparations from gram-positive bacteria, the presence of LTA, the major proinflammatory structure on these bacteria, has been known to have high alanine content, we investigated the responsiveness of both strains to anti-LTA antibody by flow cytometry. L-137 cells reacted more with anti-LTA antibody than did JCM1149 cells. Furthermore, derivative strains of L-137, cured of a specific plasmid pLTK11 of the 15 endogenous plasmids in wild-type L-137, had poor responsiveness to anti-LTA antibody and showed lower IL-12p40 inducing activity than the wild-type L-137 with pLTK11. Our results suggest that LTA expression on the cell surface causes IL-12p40 induction, and that the above internal plasmid of L-137 influences LTA synthesis and expression on the cell surface. |
| Volume | 54(3) |
| Pages | 143-51 |
| Published | 2010-3-1 |
| DOI | 10.1111/j.1348-0421.2009.00189.x |
| PII | MIM189 |
| PMID | 20236424 |
| MeSH | Animals Cell Wall / chemistry Cell Wall / immunology* Female Interleukin-12 Subunit p40 / genetics Interleukin-12 Subunit p40 / immunology* Lactobacillus plantarum / chemistry Lactobacillus plantarum / genetics Lactobacillus plantarum / immunology* Lipopolysaccharides / genetics Lipopolysaccharides / immunology* Mice Mice, Inbred BALB C Models, Animal Teichoic Acids / genetics Teichoic Acids / immunology* |
| IF | 1.566 |
| Times Cited | 18 |
| WOS Category | MICROBIOLOGY IMMUNOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 11 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| General Microbes | JCM 1149 |