Reference - Detail
| RRC ID | 71538 |
|---|---|
| Author | Okuma N, Soyano T, Suzaki T, Kawaguchi M. |
| Title | MIR2111-5 locus and shoot-accumulated mature miR2111 systemically enhance nodulation depending on HAR1 in Lotus japonicus. |
| Journal | Nat Commun |
| Abstract |
Legumes utilize a shoot-mediated signaling system to maintain a mutualistic relationship with nitrogen-fixing bacteria in root nodules. In Lotus japonicus, shoot-to-root transfer of microRNA miR2111 that targets TOO MUCH LOVE, a nodulation suppressor in roots, has been proposed to explain the mechanism underlying nodulation control from shoots. However, the role of shoot-accumulating miR2111s for the systemic regulation of nodulation was not clearly shown. Here, we find L. japonicus has seven miR2111 loci, including those mapped through RNA-seq. MIR2111-5 expression in leaves is the highest among miR2111 loci and repressed after rhizobial infection depending on a shoot-acting HYPERNODULATION ABERRANT ROOT FORMATION1 (HAR1) receptor. MIR2111-5 knockout mutants show significantly decreased nodule numbers and miR2111 levels. Furthermore, grafting experiments using transformants demonstrate scions with altered miR2111 levels influence nodule numbers in rootstocks in a dose-dependent manner. Therefore, miR2111 accumulation in leaves through MIR2111-5 expression is required for HAR1-dependent systemic optimization of nodule number. |
| Volume | 11(1) |
| Pages | 5192 |
| Published | 2020-10-15 |
| DOI | 10.1038/s41467-020-19037-9 |
| PII | 10.1038/s41467-020-19037-9 |
| PMID | 33060582 |
| PMC | PMC7562733 |
| MeSH | Gene Expression Regulation, Plant Gene Knockout Techniques Lotus / genetics Lotus / metabolism* MicroRNAs / genetics MicroRNAs / metabolism* Plant Leaves Plant Proteins / genetics Plant Proteins / metabolism* Plant Root Nodulation / physiology* Plant Roots / metabolism Plant Shoots / genetics Plant Shoots / metabolism* Rhizobium / metabolism Root Nodules, Plant / growth & development* Sequence Analysis Signal Transduction / physiology Transcriptome |
| IF | 12.121 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 11 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Lotus / Glycine | MG20 |