論文 - 詳細
| RRC ID | 71604 |
|---|---|
| 著者 | Liu SQ, Shiba R, Kim BS, Saijo K, Ohno T. |
| タイトル | Long-term serum/plasma-free culture of human cytotoxic T lymphocytes induced from peripheral blood mononuclear cells. |
| ジャーナル | Cancer Immunol Immunother |
| Abstract |
Tumor-specific human cytotoxic T lymphocytes (CTL) were induced by co-culturing peripheral blood mononuclear cells with X-ray-irradiated human lung squamous carcinoma cells, SQ-5, in the medium supplemented with interleukin(IL)-1, IL-2, IL-4 and IL-6, and 5% autologous plasma for 3 or 5 days. The CTL grew in serum/plasma-free medium containing these four interleukins and 0.5% bovine serum albumin for over a month and maintained killing activity of target cells within 48 h at an effector/target ratio of 1.25. Their growth was essentially dependent on the target SQ-5 cells, which were renewed every 5 days. Under these conditions, IL-4 and IL-6 could be omitted. When anti-CD3 monoclonal antibody was added to the serum/plasma-free medium supplemented with IL-1 and IL-2, the target tumor cells were not required to maintain the specific killing activity of the CTL. A large number of CTL (10(11)) were obtained in 35 days. |
| 巻・号 | 39(5) |
| ページ | 279-85 |
| 公開日 | 1994-11-1 |
| DOI | 10.1007/BF01519979 |
| PMID | 7987858 |
| PMC | PMC11038377 |
| MeSH | Carcinoma, Squamous Cell / pathology Cells, Cultured Culture Media, Serum-Free Cytotoxicity, Immunologic Humans Immunotherapy, Adoptive Leukocytes, Mononuclear / immunology Lung Neoplasms / pathology T-Lymphocytes, Cytotoxic / immunology* T-Lymphocytes, Cytotoxic / physiology Tumor Cells, Cultured |
| IF | 5.442 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | A549(RCB0098) K562(RCB0027) RERF-LC-AI(RCB0444) |