| RRC ID |
71604
|
| Author |
Liu SQ, Shiba R, Kim BS, Saijo K, Ohno T.
|
| Title |
Long-term serum/plasma-free culture of human cytotoxic T lymphocytes induced from peripheral blood mononuclear cells.
|
| Journal |
Cancer Immunol Immunother
|
| Abstract |
Tumor-specific human cytotoxic T lymphocytes (CTL) were induced by co-culturing peripheral blood mononuclear cells with X-ray-irradiated human lung squamous carcinoma cells, SQ-5, in the medium supplemented with interleukin(IL)-1, IL-2, IL-4 and IL-6, and 5% autologous plasma for 3 or 5 days. The CTL grew in serum/plasma-free medium containing these four interleukins and 0.5% bovine serum albumin for over a month and maintained killing activity of target cells within 48 h at an effector/target ratio of 1.25. Their growth was essentially dependent on the target SQ-5 cells, which were renewed every 5 days. Under these conditions, IL-4 and IL-6 could be omitted. When anti-CD3 monoclonal antibody was added to the serum/plasma-free medium supplemented with IL-1 and IL-2, the target tumor cells were not required to maintain the specific killing activity of the CTL. A large number of CTL (10(11)) were obtained in 35 days.
|
| Volume |
39(5)
|
| Pages |
279-85
|
| Published |
1994-11-1
|
| DOI |
10.1007/BF01519979
|
| PMID |
7987858
|
| PMC |
PMC11038377
|
| MeSH |
Carcinoma, Squamous Cell / pathology
Cells, Cultured
Culture Media, Serum-Free
Cytotoxicity, Immunologic
Humans
Immunotherapy, Adoptive
Leukocytes, Mononuclear / immunology
Lung Neoplasms / pathology
T-Lymphocytes, Cytotoxic / immunology*
T-Lymphocytes, Cytotoxic / physiology
Tumor Cells, Cultured
|
| IF |
5.442
|
| Resource |
| Human and Animal Cells |
A549(RCB0098)
K562(RCB0027)
RERF-LC-AI(RCB0444) |
