RRC ID 71604
Author Liu SQ, Shiba R, Kim BS, Saijo K, Ohno T.
Title Long-term serum/plasma-free culture of human cytotoxic T lymphocytes induced from peripheral blood mononuclear cells.
Journal Cancer Immunol Immunother
Abstract Tumor-specific human cytotoxic T lymphocytes (CTL) were induced by co-culturing peripheral blood mononuclear cells with X-ray-irradiated human lung squamous carcinoma cells, SQ-5, in the medium supplemented with interleukin(IL)-1, IL-2, IL-4 and IL-6, and 5% autologous plasma for 3 or 5 days. The CTL grew in serum/plasma-free medium containing these four interleukins and 0.5% bovine serum albumin for over a month and maintained killing activity of target cells within 48 h at an effector/target ratio of 1.25. Their growth was essentially dependent on the target SQ-5 cells, which were renewed every 5 days. Under these conditions, IL-4 and IL-6 could be omitted. When anti-CD3 monoclonal antibody was added to the serum/plasma-free medium supplemented with IL-1 and IL-2, the target tumor cells were not required to maintain the specific killing activity of the CTL. A large number of CTL (10(11)) were obtained in 35 days.
Volume 39(5)
Pages 279-85
Published 1994-11-1
DOI 10.1007/BF01519979
PMID 7987858
MeSH Carcinoma, Squamous Cell / pathology Cells, Cultured Culture Media, Serum-Free Cytotoxicity, Immunologic Humans Immunotherapy, Adoptive Leukocytes, Mononuclear / immunology Lung Neoplasms / pathology T-Lymphocytes, Cytotoxic / immunology* T-Lymphocytes, Cytotoxic / physiology Tumor Cells, Cultured
IF 5.442
Human and Animal Cells A549(RCB0098) K562(RCB0027) RERF-LC-AI(RCB0444)