論文 - 詳細
| RRC ID | 71608 |
|---|---|
| 著者 | Eguchi S, Kimura K, Kageyama K, Tani N, Tanaka R, Nishio K, Shinkawa H, Ohira GO, Amano R, Tanaka S, Yamamoto A, Takemura S, Yashiro M, Kubo S. |
| タイトル | Optimal Organ for Patient-derived Xenograft Model in Pancreatic Cancer and Microenvironment that Contributes to Success. |
| ジャーナル | Anticancer Res |
| Abstract |
BACKGROUND:We aimed to investigate the difference in engraftment rates depending on the transplant site for a patient-derived xenograft (PDX) of pancreatic ductal adenocarcinoma (PDAC) and the effects of the microenvironment on engraftment. MATERIALS AND METHODS:Frozen cancer tissues from PDAC tumors were used, and tumor fragments were directly implanted into the subcutaneous, orthotopic pancreas, peritoneum, and liver of X-linked severe combined immunodeficiency (XSCID) rats. We assessed the success of engraftment in each organ. Additionally, to evaluate the effect of the microenvironment in each organ, we performed immunohistochemical analysis. RESULTS:Subcutaneous transplantation was successful in 8 of 10 PDAC cases (16 of 30 rats). This was a higher rate than for other organ transplants. The vascular endothelial cells in the stroma were replaced with those from rats instead of humans. Vascular endothelial growth factor-A (VEGF-A) and cluster of differentiation-31 (CD31) was significantly more strongly expressed in the subcutaneous transplantation model (VEGF-A: p<0.001, CD31: p=0.0036). CONCLUSION:The engraftment rate was significantly higher for the subcutaneous PDX model than for the orthotopic pancreatic, peritoneal, and liver PDX models. Blood vessels of the PDX stroma had been replaced by rat-derived vessels instead of the original human vessels, suggesting that angiogenesis in the PDX microenvironment may be a major factor in engraftment. |
| 巻・号 | 42(5) |
| ページ | 2395-2404 |
| 公開日 | 2022-5-1 |
| DOI | 10.21873/anticanres.15718 |
| PII | 42/5/2395 |
| PMID | 35489770 |
| MeSH | Animals Carcinoma, Pancreatic Ductal* / pathology Disease Models, Animal Endothelial Cells / pathology Heterografts Humans Pancreatic Neoplasms* / pathology Rats Tumor Microenvironment Vascular Endothelial Growth Factor A Xenograft Model Antitumor Assays |
| IF | 1.994 |
| リソース情報 | |
| ラット | X-linked severe combined immunodeficiency (XSCID) rats |