| RRC ID |
71639
|
| 著者 |
Kawatani M, Aono H, Hiranuma S, Shimizu T, Muroi M, Ogawa N, Ohishi T, Ohba SI, Kawada M, Nogawa T, Okano A, Hashizume D, Osada H.
|
| タイトル |
Identification of a Small-Molecule Glucose Transporter Inhibitor, Glutipyran, That Inhibits Cancer Cell Growth.
|
| ジャーナル |
ACS Chem Biol
|
| Abstract |
Cancer cells reprogram their metabolism to survive and grow. Small-molecule inhibitors targeting cancer are useful for studying its metabolic pathways and functions and for developing anticancer drugs. Here, we discovered that glutipyran and its derivatives inhibit glycolytic activity and cell growth in human pancreatic cancer cells. According to proteomic profiling of glutipyran-treated cells using our ChemProteoBase, glutipyran was clustered within the group of endoplasmic reticulum (ER) stress inducers that included glycolysis inhibitors. Glutipyran inhibited glucose uptake and suppressed the growth of various cancer cells, including A431 cells that express glucose transporter class I (GLUT1) and DLD-1 GLUT1 knockout cells. When cotreated with the mitochondrial respiration inhibitor metformin, glutipyran exhibited a synergistic antiproliferative effect. Metabolome analysis revealed that glutipyran markedly decreased most metabolites of the glycolytic pathway and the pentose phosphate pathway. Glutipyran significantly suppressed tumor growth in a xenograft mouse model of pancreatic cancer. These results suggest that glutipyran acts as a broad-spectrum GLUT inhibitor and reduces cancer cell growth.
|
| 巻・号 |
16(8)
|
| ページ |
1576-1586
|
| 公開日 |
2021-8-20
|
| DOI |
10.1021/acschembio.1c00480
|
| PMID |
34296611
|
| MeSH |
Animals
Antineoplastic Agents / chemical synthesis
Antineoplastic Agents / pharmacology
Antineoplastic Agents / therapeutic use*
Cell Line, Tumor
Cell Proliferation / drug effects
Drug Screening Assays, Antitumor
Drug Synergism
Female
Glucose / metabolism
Glucose Transport Proteins, Facilitative / antagonists & inhibitors*
Glycolysis / drug effects
Humans
Metabolomics
Metformin / therapeutic use
Mice, Inbred BALB C
Mice, Nude
Neoplasms / drug therapy*
Proteomics
Pyrans / chemical synthesis
Pyrans / pharmacology
Pyrans / therapeutic use*
Xenograft Model Antitumor Assays
|
| IF |
4.434
|
| リソース情報 |
| ヒト・動物細胞 |
HeLa
A549
MCF7(RCB1904)
MKN74(RCB1002)
A431
PANC-1(RCB2095)
MIA Paca2(RCB2094)
1B2C6(RCB0795)
1C3D3(RCB0796) |
