RRC ID 71646
Author Kohno T, Konno T, Kikuchi S, Kondoh M, Kojima T.
Title Translocation of LSR from tricellular corners causes macropinocytosis at cell-cell interface as a trigger for breaking out of contact inhibition.
Journal FASEB J
Abstract Withdrawal from contact inhibition is necessary for epithelial cancer precursor cells to initiate cell growth and motility. Nevertheless, little is understood about the mechanism for the sudden initiation of cell growth under static conditions. We focused on cellular junctions as one region where breaking out of contact inhibition occurs. In well-differentiated endometrial cancer cells, Sawano, the ligand administration for tricellular tight junction protein LSR, which transiently decreased the robust junction property, caused an abrupt increase in cell motility and consequent excessive multilayered cell growth despite being under contact inhibition conditions. We observed that macropinocytosis essentially and temporarily occurred as an antecedent event for the above process at intercellular junctions without disruption of the junction apparatus but not at the apical plasma membrane. Collectively, we concluded that the formation of macropinocytosis, which is derived from tight junction-mediated signaling, was triggered for the initiation of cell growth in static precancerous epithelium.
Volume 35(9)
Pages e21742
Published 2021-9-1
DOI 10.1096/fj.202100299R
PMID 34403506
MeSH Bacterial Toxins / pharmacology Binding Sites Cell Adhesion* Cell Growth Processes / drug effects Cell Line, Tumor Cell Movement / drug effects Contact Inhibition* Humans Intercellular Junctions / drug effects Intercellular Junctions / metabolism JNK Mitogen-Activated Protein Kinases / metabolism Phenotype Pinocytosis* / drug effects Protein Transport Receptors, Lipoprotein / metabolism* Transcription Factors / metabolism* Vacuoles / drug effects Vacuoles / metabolism rac GTP-Binding Proteins / metabolism
IF 4.966
Resource
Human and Animal Cells Sawano(RCB1152)