| RRC ID |
71651
|
| 著者 |
Miyagawa Y, Ohguro H, Odagiri H, Maruyama I, Maeda T, Maeda A, Sasaki M, Nakazawa M.
|
| タイトル |
Aberrantly expressed recoverin is functionally associated with G-protein-coupled receptor kinases in cancer cell lines.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
Cancer-associated retinopathy (CAR) is an ocular manifestation of a paraneoplastic syndrome whereby immunological reactions toward recoverin (Rec), a retina-specific Ca(2+) binding protein, and its aberrant expression in tumor cells lead to the retinal degeneration. To elucidate functional roles of the aberrantly expression in cancer cells, we performed immunoprecipitation using anti-human Rec mAb. We observed co-precipitation of G-protein-coupled receptor kinases (GRKs) and caveolin-1 with Rec from cell lysates of 293 or SSTW cells. Immunocytochemistry revealed that immunoreactivities toward Rec within the cancer cells were almost identical to those toward GRKs and caveolin-1. The present data strongly suggest that aberrantly expressed Rec should be involved in the GRK-dependent cellular regulation in cancer cells.
|
| 巻・号 |
300(3)
|
| ページ |
669-73
|
| 公開日 |
2003-1-17
|
| DOI |
10.1016/s0006-291x(02)02888-7
|
| PII |
S0006291X02028887
|
| PMID |
12507501
|
| MeSH |
Antibodies, Monoclonal / pharmacology
Calcium-Binding Proteins / antagonists & inhibitors
Calcium-Binding Proteins / metabolism*
Caveolin 1
Caveolins / metabolism
Cell Line
Cyclic AMP-Dependent Protein Kinases / metabolism*
Eye Proteins*
G-Protein-Coupled Receptor Kinase 2
G-Protein-Coupled Receptor Kinase 5
G-Protein-Coupled Receptor Kinases
GTP-Binding Proteins / metabolism
Hippocalcin
Humans
Immunohistochemistry
Kidney / cytology
Kidney / metabolism
Lipoproteins*
Nerve Tissue Proteins*
Precipitin Tests
Protein Binding
Protein Serine-Threonine Kinases / metabolism*
Recoverin
Stomach Neoplasms / metabolism*
Stomach Neoplasms / pathology
Tumor Cells, Cultured
beta-Adrenergic Receptor Kinases
|
| IF |
2.985
|
| リソース情報 |
| ヒト・動物細胞 |
293(RCB1637) |
