RRC ID 71652
Author Matsuoka K, Isowa N, Yoshimura T, Liu M, Wada H.
Title Green tea polyphenol blocks h(2)o(2)-induced interleukin-8 production from human alveolar epithelial cells.
Journal Cytokine
Abstract Reactive oxygen species (ROS) play crucial roles in ischemia-reperfusion (IR) injury of lung transplants. Reactive oxygen species may stimulate the production of neutrophil chemotactic factors such as interleukin-8 (IL-8), from alveolar epithelial cells, causing recruitment and activation of neutrophils in the reperfused tissue. Green tea polyphenol has potent anti-oxidative activities and anti-inflammatory effects by decreasing cytokine production. In the present study, we found that green tea polyphenol significantly inhibited IL-8 production induced by hydrogen peroxide (H(2)O(2)) in human lung alveolar epithelial cells (A549 line). It has been shown that mitogen activated protein kinases, such as Jun N-terminal kinase (JNK), p38 and p44/42, could mediate IL-8 production from a variety of cell types. We further investigated the effect of green tea polyphenol on these protein kinases, and demonstrated that H(2)O(2)-induced phosphorylation of JNK and p38 but not p44/42 was inhibited by green tea polyphenol in A549 cells. We speculate that green tea polyphenol may inhibit H(2)O(2)-induced IL-8 production from A549 cells through inactivation of JNK and p38.
Volume 18(5)
Pages 266-73
Published 2002-6-7
DOI 10.1006/cyto.2002.1038
PII S1043466602910380
PMID 12161102
MeSH Antioxidants / pharmacology Blotting, Western Cell Line Dose-Response Relationship, Drug Enzyme Activation / drug effects Epithelial Cells / metabolism Epithelium / metabolism* Flavonoids* Humans Hydrogen Peroxide / pharmacology* Interleukin-8 / biosynthesis* Lung / cytology* Lung / metabolism* Perfusion Phenols / pharmacology* Polymers / pharmacology* Polyphenols RNA, Messenger / metabolism Reverse Transcriptase Polymerase Chain Reaction Tea / metabolism* Time Factors
IF 2.952
Human and Animal Cells A549(RCB0098)