RRC ID 717
Author Hoshino A, Morita Y, Choi JD, Saito N, Toki K, Tanaka Y, Iida S.
Title Spontaneous mutations of the flavonoid 3'-hydroxylase gene conferring reddish flowers in the three morning glory species.
Journal Plant Cell Physiol
Abstract Among the Ipomoea plants, both Ipomoea nil and Ipomoea tricolor display bright blue flowers, and Ipomoea purpurea exhibits dark purple flowers. While all of these flowers contain cyanidin-based anthocyanin pigments, the mutants of I. nil, I. purpurea, and I. tricolor carrying the magenta, pink, and fuchsia alleles, respectively, produce reddish flowers containing pelargonidin derivatives, and all of them are deficient in the gene for flavonoid 3'-hydroxylase (F3'H). The magenta allele in I. nil is a nonsense mutation caused by a single C to T base transition generating the stop codon TGA, and the cultivar Violet carries the same mutation. Several tested pink mutants in I. purpurea carry inserts of the 0.55-kb DNA transposable element Tip201 belonging to the Ac/Ds superfamily at the identical site. No excision of Tip201 from the F3'H gene could be detected, and both splicing and polyadenylation patterns of the F3'H transcripts were affected by the Tip201 integration. The fuchsia allele in I. tricolor is a single T insertion generating the stop codon TAG, and the accumulation of the F3'H transcripts was drastically reduced by the nonsense-mediated RNA decay. Spontaneous mutations in Ipomoea, including a possible founder mutation in the pink allele, are also discussed.
Volume 44(10)
Pages 990-1001
Published 2003-10-1
DOI 10.1093/pcp/pcg143
PMID 14581624
MeSH Amino Acid Sequence Base Sequence Cytochrome P-450 Enzyme System / genetics* DNA Primers Flowers / genetics* Gene Expression Regulation, Plant Genes, Plant Ipomoea / enzymology Ipomoea / genetics* Mixed Function Oxygenases / genetics* Molecular Sequence Data Mutation* Pigmentation / genetics*
IF 4.062
Times Cited 50
Morning Glory AK5 AK26 AK29 AK33 AK36 AK37 AK38 AK39 AK43 AK47 AK48 AK49 AK52 AK61 AK62 AK70 AK301 AK302 AK303 AK304 AK305 AK306 AK311