RRC ID 71708
Author Hino M, Kurogi K, Okubo MA, Murata-Hori M, Hosoya H.
Title Small heat shock protein 27 (HSP27) associates with tubulin/microtubules in HeLa cells.
Journal Biochem Biophys Res Commun
Abstract One of the monoclonal antibodies raised against mitotic HeLa cells (termed as mH3) recognized a 27-kDa protein and stained microtubules in the mitotic spindles of HeLa cells. Immunoscreening of a HeLa cDNA library revealed that mH3 antigen is a small heat shock protein, HSP27. Immunoprecipitation analysis using mH3 suggested that both alpha- and beta-tubulin are associated with HSP27. Further, sucrose-cushioned ultra centrifugation revealed that HSP27 is co-sedimented with taxol-stabilized microtubules. These results indicate that HSP27 associates with tubulin/microtubules in HeLa cells.
Volume 271(1)
Pages 164-9
Published 2000-4-29
DOI 10.1006/bbrc.2000.2553
PII S0006-291X(00)92553-1
PMID 10777697
MeSH Amino Acid Sequence Antibodies, Monoclonal / metabolism Fluorescent Antibody Technique, Indirect HeLa Cells Heat-Shock Proteins / metabolism* Humans Microscopy, Confocal Microtubules / metabolism* Mitosis Molecular Sequence Data Precipitin Tests Protein Binding Sequence Homology, Amino Acid Tubulin / metabolism*
IF 2.985
Human and Animal Cells HeLa(RCB0007)