RRC ID |
71721
|
著者 |
Nagai M, Kyakumoto S, Sato N.
|
タイトル |
Cancer cells responsible for humoral hypercalcemia express mRNA encoding a secreted form of ODF/TRANCE that induces osteoclast formation.
|
ジャーナル |
Biochem Biophys Res Commun
|
Abstract |
A novel cDNA encoding a secreted form of osteoclast differentiation factor/tumor necrosis factor-related activation-induced cytokine (sODF/TRANCE, GenBank Accession No. AB037599) was sequenced from 5' RACE cDNA clones of squamous cell carcinoma cell lines, SCC-4 and T3M-1 Cl.2, of which parental malignant tissues had caused severe humoral hypercalcemia. The sODF/TRANCE cDNA was composed of unknown 5' end sequence followed by the 100% identical sequence of the ODF/TRANCE extracellular domain-coding region. The longest open reading frame (ORF) of the novel cDNA completely matched the 3' end of the ORF of the ODF/TRANCE cDNA encoding C-terminal amino acid residues (74-318) in the extracellular region. The corresponding protein that reacted with the antibody specific for the extracellular domain of ODF/TRANCE was detected in the culture media conditioned by the cancer cells. Furthermore, human promyeloblastic leukemia cells, HL60, differentiated into osteoclast-like cells (OCLs) when cultured in the media conditioned by SCC-4 and T3M-1 Cl. 2 cells. The differentiation of HL60 cells into OCLs was inhibited by the anti-ODF/TRANCE antibody. These results strongly suggest that sODF/TRANCE plays an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy.
|
巻・号 |
269(2)
|
ページ |
532-6
|
公開日 |
2000-3-16
|
DOI |
10.1006/bbrc.2000.2314
|
PII |
S0006-291X(00)92314-3
|
PMID |
10708588
|
MeSH |
Amino Acid Sequence
Base Sequence
Carrier Proteins / genetics*
Cells, Cultured
DNA, Complementary
Humans
Hypercalcemia / etiology*
Immunohistochemistry
Membrane Glycoproteins / genetics*
Molecular Sequence Data
Neoplasms / complications*
Osteoclasts / cytology*
RANK Ligand
RNA, Messenger / genetics*
Receptor Activator of Nuclear Factor-kappa B
Tumor Cells, Cultured
Tumor Necrosis Factor-alpha / genetics
|
IF |
2.985
|
リソース情報 |
ヒト・動物細胞 |
T3M-1 Clone2(RCB1015)
HFL-I(RCB0521)
HL60(RCB0041) |