| RRC ID |
71722
|
| Author |
Liu SQ, Saijo K, Todoroki T, Ohno T.
|
| Title |
Induction of human autologous cytotoxic T lymphocytes on formalin-fixed and paraffin-embedded tumour sections.
|
| Journal |
Nat Med
|
| Abstract |
Human autologous tumour-specific cytotoxic T lymphocytes (CTL) were generated from peripheral blood on small formalin-fixed paraffin-embedded sections of a gastric cancer. The CTL killed live target cells at an effector/target ratio of 1 within 24 hours and showed the same target specificity as those induced on live cancer cells. The killing activity of the CTL lasted for more than four months in culture and was inhibited by antibodies against CD8 and MHC-class I. These results suggest that adoptive immunotherapy of tumours will be possible with CTL induced on a stable source of tumour antigen.
|
| Volume |
1(3)
|
| Pages |
267-71
|
| Published |
1995-3-1
|
| DOI |
10.1038/nm0395-267
|
| PMID |
7585045
|
| MeSH |
Adenocarcinoma / immunology
Antigens, Neoplasm / chemistry
Antigens, Neoplasm / immunology*
Cells, Cultured
Cytotoxicity, Immunologic
Formaldehyde / chemistry
Humans
Immunophenotyping
Killer Cells, Lymphokine-Activated / immunology
Killer Cells, Natural / immunology
Lymphocytes, Tumor-Infiltrating / immunology
Middle Aged
Paraffin
Stomach Neoplasms / immunology
T-Lymphocytes, Cytotoxic / immunology*
Tumor Cells, Cultured
|
| IF |
36.13
|
| Resource |
| Human and Animal Cells |
K562(RCB0027) |
