RRC ID 7173
著者 Hozumi A, Kawai N, Yoshida R, Ogura Y, Ohta N, Satake H, Satoh N, Sasakura Y.
タイトル Efficient transposition of a single Minos transposon copy in the genome of the ascidian Ciona intestinalis with a transgenic line expressing transposase in eggs.
ジャーナル Dev Dyn
Abstract Transgenesis with transposons is an important technique for studying genetic functions. In the ascidian Ciona intestinalis, methods for germline transformation with the Tc1/mariner transposon Minos have been established. A system to remobilize a single Minos copy in the genome is needed to refine this transgenic technique. In this study, such an experimental system was established with a transgenic line expressing Minos transposase in eggs. In the eggs of a double transgenic animal from a cross between the egg transposase line and a transgenic line having a single Minos insertion, the transposon was transposed into new positions of the Ciona genome, thus creating new insertions. Some of the new insertions caused enhancer detection. The majority of the new insertion sites were mapped on different chromosomes from that of the transposon donor. This characteristic of Minos is in contrast to that of the Sleeping Beauty transposon, which causes frequent intrachromosomal transposition.
巻・号 239(4)
ページ 1076-88
公開日 2010-4-1
DOI 10.1002/dvdy.22254
PMID 20186916
MeSH Animals Animals, Genetically Modified Base Sequence Ciona intestinalis / genetics* Ciona intestinalis / metabolism DNA Transposable Elements / genetics* Efficiency Embryo, Nonmammalian Gene Dosage Gene Expression Regulation, Developmental Genome Models, Biological Mutagenesis, Insertional* / genetics Mutagenesis, Insertional* / physiology Ovum / enzymology Ovum / metabolism* Transposases / genetics Transposases / metabolism* Urochordata / genetics Urochordata / metabolism
IF 3.275
引用数 28
WOS 分野 ANATOMY & MORPHOLOGY DEVELOPMENTAL BIOLOGY
リソース情報
カタユウレイボヤ・(ニッポンウミシダ) Ju[SBFr3dTPORCiNutMiTP]1