Reference - Detail
| RRC ID | 71736 |
|---|---|
| Author | Pruthvi V, Narasimhan R, Nataraja KN. |
| Title | Simultaneous expression of abiotic stress responsive transcription factors, AtDREB2A, AtHB7 and AtABF3 improves salinity and drought tolerance in peanut (Arachis hypogaea L.). |
| Journal | PLoS One |
| Abstract |
Drought, salinity and extreme temperatures are the most common abiotic stresses, adversely affecting plant growth and productivity. Exposure of plants to stress activates stress signalling pathways that induce biochemical and physiological changes essential for stress acclimation. Stress tolerance is governed by multiple traits, and importance of a few traits in imparting tolerance has been demonstrated. Under drought, traits linked to water mining and water conservation, water use efficiency and cellular tolerance (CT) to desiccation are considered to be relevant. In this study, an attempt has been made to improve CT in drought hardy crop, peanut (Arachis hypogaea L., cv. TMV2) by co-expressing stress-responsive transcription factors (TFs), AtDREB2A, AtHB7 and AtABF3, associated with downstream gene expression. Transgenic plants simultaneously expressing these TFs showed increased tolerance to drought, salinity and oxidative stresses compared to wild type, with an increase in total plant biomass. The transgenic plants exhibited improved membrane and chlorophyll stability due to enhanced reactive oxygen species scavenging and osmotic adjustment by proline synthesis under stress. The improvement in stress tolerance in transgenic lines were associated with induced expression of various CT related genes like AhGlutaredoxin, AhAldehyde reductase, AhSerine threonine kinase like protein, AhRbx1, AhProline amino peptidase, AhHSP70, AhDIP and AhLea4. Taken together the results indicate that co-expression of stress responsive TFs can activate multiple CT pathways, and this strategy can be employed to improve abiotic stress tolerance in crop plants. |
| Volume | 9(12) |
| Pages | e111152 |
| Published | 2014-1-1 |
| DOI | 10.1371/journal.pone.0111152 |
| PII | PONE-D-13-54106 |
| PMID | 25474740 |
| PMC | PMC4256372 |
| MeSH | Acclimatization / genetics Arabidopsis / genetics Arabidopsis Proteins / biosynthesis Arabidopsis Proteins / genetics* Arachis / genetics* Arachis / physiology Basic-Leucine Zipper Transcription Factors / biosynthesis Basic-Leucine Zipper Transcription Factors / genetics* Chlorophyll / genetics Droughts* Gene Expression Regulation, Plant Oxidative Stress / genetics Plant Leaves / genetics Plant Leaves / growth & development Plants, Genetically Modified / genetics Plants, Genetically Modified / growth & development Reactive Oxygen Species / metabolism Salinity Seedlings / genetics Seedlings / growth & development Stress, Physiological / genetics* Transcription Factors / biosynthesis Transcription Factors / genetics* |
| IF | 2.74 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Arabidopsis / Cultured plant cells, genes | pda03329 pda01352 pda02353 |