RRC ID 7174
Author Ohta N, Horie T, Satoh N, Sasakura Y.
Title Transposon-mediated enhancer detection reveals the location, morphology and development of the cupular organs, which are putative hydrodynamic sensors, in the ascidian Ciona intestinalis.
Journal Zool. Sci.
Abstract The adult of the ascidian Ciona intestinalis has cupular organs, i.e., putative hydrodynamic sensors, at the atrial epithelium. The cupular organ consists of support cells and sensory neurons, and it extends a gelatinous matrix, known as a cupula, toward the atrial cavity. These characteristics are shared with sensory hair cells in the vertebrate inner ear and lateral line neuromasts in fish and amphibians, which suggests an evolutionary link between the cupular organ and these vertebrate hydrodynamic sensors. In the present study, we have isolated and investigated two transposon-mediated enhancer detection lines that showed GFP expression in support cells of the cupular organs. Using the enhancer detection lines and neuron marker transgenic lines, we describe the position, morphology, and development of the cupular organs. Cupular organs were found at the atrial epithelium, but not in the branchial epithelium. We found that cupular organs are also present along the dorsal fold and the gonoducts. The cells lining the pre-atrial opening in juveniles are presumably precursor cells of the cupular organ. To our knowledge, the present study is the first precise description of the ascidian cupular organ, providing evidence that may help to resolve discrepancies among previous studies on the organ.
Volume 27(11)
Pages 842-50
Published 2010-11
DOI 10.2108/zsj.27.842
PMID 21039122
MeSH Animals Ciona intestinalis / anatomy & histology Ciona intestinalis / genetics Ciona intestinalis / growth & development* Ciona intestinalis / physiology* DNA Transposable Elements / genetics DNA Transposable Elements / physiology* Gene Expression Regulation, Developmental / physiology Green Fluorescent Proteins Organisms, Genetically Modified Pressure Sense Organs / growth & development* Sense Organs / physiology*
IF 0.846
Times Cited 4
WOS Category ZOOLOGY
Resource
C.intestinalis / (O.japonicus) E[MiTSAdTPOG]10 EJ[MiTSAdTPOG]62