論文 - 詳細
| RRC ID | 71752 |
|---|---|
| 著者 | Chen JR, Lazarenko OP, Blackburn ML, Chen JF, Randolph CE, Zabaleta J, Schroder K, Pedersen KB, Ronis MJJ. |
| タイトル | Nox4 expression in osteo-progenitors controls bone development in mice during early life. |
| ジャーナル | Commun Biol |
| Abstract |
Tightly regulated and cell-specific NADPH-oxidases (Nox) represent one of the major sources of reactive oxygen species (ROS) signaling molecules that are involved in tissue development and stem cell self-renewal. We have characterized the role of Nox4 in osteo-progenitors during postnatal bone development. Nox4 expression in bone and ROS generation were increased during early osteoblast differentiation and bone development. Stromal osteoblastic cell self-renewal, proliferation and ROS production were significantly lower in samples from whole-body Nox4 knockout mice (Nox4-/-) and conditional knockout (CKO) mice with depletion of Nox4 in the limb bud mesenchyme compared with those from control mice (Nox4fl/fl), but they were reversed after 9 passages. In both sexes, bone volume, trabecular number and bone mineral density were significantly lower in 3-week old CKO and Nox4-/- mice compared with Nox4fl/fl controls. This was reflected in serum levels of bone formation markers alkaline phosphatase (ALP) and procollagen 1 intact N-terminal propeptide (P1NP). However, under-developed bone formation in 3-week old CKO and Nox4-/- mice quickly caught up to levels of control mice by 6-week of age, remained no different at 13-week of age, and was reversed in 32-week old male mice. Osteoclastogenesis showed no differences among groups, however, CTX1 reflecting osteoclast activity was significantly higher in 3-week old male CKO and Nox4-/- mice compared with control mice, and significantly lower in 32-week old Nox4-/- mice compared with control mice. These data suggest that Nox4 expression and ROS signaling in bone and osteoblastic cells coordinately play an important role in osteoblast differentiation, proliferation and maturation. |
| 巻・号 | 5(1) |
| ページ | 583 |
| 公開日 | 2022-6-14 |
| DOI | 10.1038/s42003-022-03544-0 |
| PII | 10.1038/s42003-022-03544-0 |
| PMID | 35701603 |
| PMC | PMC9198054 |
| MeSH | Animals Bone Development* / physiology Female Male Mice Mice, Knockout NADPH Oxidase 4* / biosynthesis NADPH Oxidase 4* / genetics NADPH Oxidase 4* / metabolism Osteogenesis* / physiology Reactive Oxygen Species / metabolism |
| IF | 4.165 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | ST2(RCB0224) |