論文 - 詳細
| RRC ID | 71810 |
|---|---|
| 著者 | Masuda A, Lee JM, Miyata T, Ebihara T, Kakino K, Hino M, Fujita R, Mon H, Kusakabe T. |
| タイトル | Stable trimer formation of spike protein from porcine epidemic diarrhea virus improves the efficiency of secretory production in silkworms and induces neutralizing antibodies in mice. |
| ジャーナル | Vet Res |
| Abstract |
Porcine epidemic diarrhea virus (PEDV) is a highly infectious pathogen of watery diarrhea that causes serious economic loss to the swine industry worldwide. Especially because of the high mortality rate in neonatal piglets, a vaccine with less production cost and high protective effect against PEDV is desired. The intrinsically assembled homotrimer of spike (S) protein on the PEDV viral membrane contributing to the host cell entry is a target of vaccine development. In this study, we designed trimerized PEDV S protein for efficient production in the silkworm-baculovirus expression vector system (silkworm-BEVS) and evaluated its immunogenicity in the mouse. The genetic fusion of the trimeric motif improved the expression of S protein in silkworm-BEVS. A small-scale screening of silkworm strains to further improve the S protein productivity finally achieved the yield of about 2 mg from the 10 mL larval serum. Mouse immunization study demonstrated that the trimerized S protein could elicit strong humoral immunity, including the S protein-specific IgG in the serum. These sera contained neutralizing antibodies that can protect Vero cells from PEDV infection. These results demonstrated that silkworm-BEVS provides a platform for the production of trimeric S proteins, which are promising subunit vaccines against coronaviruses such as PEDV. |
| 巻・号 | 52(1) |
| ページ | 102 |
| 公開日 | 2021-7-7 |
| DOI | 10.1186/s13567-021-00971-5 |
| PII | 10.1186/s13567-021-00971-5 |
| PMID | 34233749 |
| PMC | PMC8261802 |
| MeSH | Animals Antibodies, Neutralizing / biosynthesis* Bombyx / growth & development Bombyx / metabolism* Larva / growth & development Larva / metabolism Mice Porcine epidemic diarrhea virus / genetics* Porcine epidemic diarrhea virus / metabolism Protein Multimerization Silk / biosynthesis* Spike Glycoprotein, Coronavirus / genetics* |
| IF | 3.357 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| カイコ | d36 f38 n22 n70 p53 a60 c51 d17 l311 n17 xa50 p62 p50 p02 |