| RRC ID |
71810
|
| 著者 |
Masuda A, Lee JM, Miyata T, Ebihara T, Kakino K, Hino M, Fujita R, Mon H, Kusakabe T.
|
| タイトル |
Stable trimer formation of spike protein from porcine epidemic diarrhea virus improves the efficiency of secretory production in silkworms and induces neutralizing antibodies in mice.
|
| ジャーナル |
Vet Res
|
| Abstract |
Porcine epidemic diarrhea virus (PEDV) is a highly infectious pathogen of watery diarrhea that causes serious economic loss to the swine industry worldwide. Especially because of the high mortality rate in neonatal piglets, a vaccine with less production cost and high protective effect against PEDV is desired. The intrinsically assembled homotrimer of spike (S) protein on the PEDV viral membrane contributing to the host cell entry is a target of vaccine development. In this study, we designed trimerized PEDV S protein for efficient production in the silkworm-baculovirus expression vector system (silkworm-BEVS) and evaluated its immunogenicity in the mouse. The genetic fusion of the trimeric motif improved the expression of S protein in silkworm-BEVS. A small-scale screening of silkworm strains to further improve the S protein productivity finally achieved the yield of about 2 mg from the 10 mL larval serum. Mouse immunization study demonstrated that the trimerized S protein could elicit strong humoral immunity, including the S protein-specific IgG in the serum. These sera contained neutralizing antibodies that can protect Vero cells from PEDV infection. These results demonstrated that silkworm-BEVS provides a platform for the production of trimeric S proteins, which are promising subunit vaccines against coronaviruses such as PEDV.
|
| 巻・号 |
52(1)
|
| ページ |
102
|
| 公開日 |
2021-7-7
|
| DOI |
10.1186/s13567-021-00971-5
|
| PII |
10.1186/s13567-021-00971-5
|
| PMID |
34233749
|
| PMC |
PMC8261802
|
| MeSH |
Animals
Antibodies, Neutralizing / biosynthesis*
Bombyx / growth & development
Bombyx / metabolism*
Larva / growth & development
Larva / metabolism
Mice
Porcine epidemic diarrhea virus / genetics*
Porcine epidemic diarrhea virus / metabolism
Protein Multimerization
Silk / biosynthesis*
Spike Glycoprotein, Coronavirus / genetics*
|
| IF |
3.357
|
| リソース情報 |
| カイコ |
d36
f38
n22
n70
p53
a60
c51
d17
l311
n17
xa50
p62
p50
p02 |
