論文 - 詳細
| RRC ID | 71812 |
|---|---|
| 著者 | Tomihara K, Satta K, Matsuzaki S, Yoshitake K, Yamamoto K, Uchiyama H, Yajima S, Futahashi R, Katsuma S, Osanai-Futahashi M, Kiuchi T. |
| タイトル | Mutations in a β-group of solute carrier gene are responsible for egg and eye coloration of the brown egg 4 (b-4) mutant in the silkworm, Bombyx mori. |
| ジャーナル | Insect Biochem Mol Biol |
| Abstract |
The brown egg 4 (b-4) is a recessive mutant in the silkworm (Bombyx mori), whose egg and adult compound eyes exhibit a reddish-brown color instead of normal purple and black, respectively. By double digest restriction-site associated DNA sequencing (ddRAD-seq) analysis, we narrowed down a region linked to the b-4 phenotype to approximately 1.1 Mb that contains 69 predicted gene models. RNA-seq analysis in a b-4 strain indicated that one of the candidate genes had a different transcription start site, which generates a short open reading frame. We also found that exon skipping was induced in the same gene due to an insertion of a transposable element in other two b-4 mutant strains. This gene encoded a putative amino acid transporter that belongs to the β-group of solute carrier (SLC) family and is orthologous to Drosophila eye color mutant gene, mahogany (mah). Accordingly, we named this gene Bmmah. We performed CRISPR/Cas9-mediated gene knockout targeting Bmmah. Several adult moths in generation 0 (G0) had totally or partially reddish-brown compound eyes. We also established three Bmmah knockout strains, all of which exhibit reddish-brown eggs and adult compound eyes. Furthermore, eggs from complementation crosses between the b-4 mutants and the Bmmah knockout mutants also exhibited reddish-brown color, which was similar to the b-4 mutant eggs, indicating that Bmmah is responsible for the b-4 phenotypes. |
| 巻・号 | 137 |
| ページ | 103624 |
| 公開日 | 2021-10-1 |
| DOI | 10.1016/j.ibmb.2021.103624 |
| PII | S0965-1748(21)00107-7 |
| PMID | 34333110 |
| MeSH | Amino Acid Sequence Animals Base Sequence Bombyx / genetics* Bombyx / growth & development Bombyx / metabolism Compound Eye, Arthropod / chemistry* Insect Proteins / chemistry Insect Proteins / genetics* Insect Proteins / metabolism Larva / growth & development Larva / metabolism Mutation Ovum / chemistry Phylogeny Pigmentation / genetics Pigments, Biological / analysis Sequence Alignment |
| IF | 3.827 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| カイコ | e36,e37 |