| RRC ID |
71819
|
| 著者 |
Ebihara T, Masuda A, Takahashi D, Hino M, Mon H, Kakino K, Fujii T, Fujita R, Ueda T, Lee JM, Kusakabe T.
|
| タイトル |
Production of scFv, Fab, and IgG of CR3022 Antibodies Against SARS-CoV-2 Using Silkworm-Baculovirus Expression System.
|
| ジャーナル |
Mol Biotechnol
|
| Abstract |
COVID-19, caused by SARS-CoV-2, is currently spreading around the world and causing many casualties. Antibodies against such emerging infectious diseases are one of the important tools for basic viral research and the development of diagnostic and therapeutic agents. CR3022 is a monoclonal antibody against the receptor binding domain (RBD) of the spike protein (S protein) of SARS-CoV found in SARS patients, but it was also shown to have strong affinity for that of SARS-CoV-2. In this study, we produced large amounts of three formats of CR3022 antibodies (scFv, Fab and IgG) with high purity using a silkworm-baculovirus expression vector system. Furthermore, SPR measurements showed that the affinity of those silkworm-produced IgG antibodies to S protein was almost the same as that produced in mammalian expression system. These results indicate that the silkworm-baculovirus expression system is an excellent expression system for emerging infectious diseases that require urgent demand for diagnostic agents and therapeutic agents.
|
| 巻・号 |
63(12)
|
| ページ |
1223-1234
|
| 公開日 |
2021-12-1
|
| DOI |
10.1007/s12033-021-00373-0
|
| PII |
10.1007/s12033-021-00373-0
|
| PMID |
34304364
|
| PMC |
PMC8310559
|
| MeSH |
Animals
Antibodies, Monoclonal / biosynthesis*
Antibodies, Monoclonal / genetics
Antibodies, Neutralizing / biosynthesis*
Antibodies, Neutralizing / genetics
Antibodies, Viral / biosynthesis*
Antibodies, Viral / genetics
Antibody Affinity
Baculoviridae / genetics
Baculoviridae / immunology
Biotechnology
Bombyx / genetics
Bombyx / immunology
COVID-19 / immunology*
COVID-19 / virology*
Cells, Cultured
Gene Expression
Hemolymph / immunology
Humans
Immunoglobulin Fab Fragments / biosynthesis
Immunoglobulin Fab Fragments / genetics
Immunoglobulin Fragments / biosynthesis
Immunoglobulin G / biosynthesis
Immunoglobulin G / genetics
SARS-CoV-2 / genetics
SARS-CoV-2 / immunology*
Single-Chain Antibodies / biosynthesis
Single-Chain Antibodies / genetics
Spike Glycoprotein, Coronavirus / genetics
Spike Glycoprotein, Coronavirus / immunology
|
| IF |
2.022
|
| リソース情報 |
| カイコ |
f38 |
