RRC ID 7187
Author Hirai T, Fukukawa G, Kakuta H, Fukuda N, Ezura H.
Title Production of recombinant miraculin using transgenic tomatoes in a closed cultivation system.
Journal J Agric Food Chem
Abstract We constructed a cultivation system with a controlled light period, light intensity, temperature, and CO(2) concentration for mass production of the taste-modifying protein miraculin from transgenic tomatoes. The tomato plants exhibited normal growth and produced over 270 g of fresh weight (FW) fruit per plant, with the recombinant miraculin concentration reaching up to 90 microg per g FW of tomatoes. The recombinant miraculin content of transgenic tomatoes was compared to that of plants grown in a netted greenhouse. The recombinant miraculin content of transgenic tomatoes grown in a closed cultivation system was more stable than that of tomatoes grown in a netted greenhouse, suggesting that the closed cultivation system is suitable for the production of recombinant miraculin. We estimate that 45 tFW of tomatoes and 4 kg of recombinant miraculin per 1,000 m(2) of cultivation area can be harvested per year.
Volume 58(10)
Pages 6096-101
Published 2010-5-26
DOI 10.1021/jf100414v
PMID 20426470
MeSH Agriculture / methods Environment, Controlled Fruit / chemistry Fruit / metabolism Glycoproteins / analysis Glycoproteins / biosynthesis* Glycoproteins / genetics Lycopersicon esculentum / genetics Lycopersicon esculentum / growth & development* Lycopersicon esculentum / metabolism* Plants, Genetically Modified / metabolism* Recombinant Proteins / analysis Recombinant Proteins / biosynthesis*
IF 4.192
Times Cited 21
Tomato TOMJPF00002(Moneymaker)