| RRC ID |
71883
|
| 著者 |
Kitamura H, Okita K, Fujikura D, Mori K, Iwanaga T, Saito M.
|
| タイトル |
Induction of Src-suppressed C kinase substrate (SSeCKS) in vascular endothelial cells by bacterial lipopolysaccharide.
|
| ジャーナル |
J Histochem Cytochem
|
| Abstract |
We isolated cDNA of the mouse homologue of the src-suppressed C kinase substrate (SSeCKS) and analyzed the effects of lipopolysaccharide (LPS) injection on the tissue expression pattern of this protein. Northern blotting analysis showed that SSeCKS mRNA was expressed abundantly in the testis but at undetectable levels in other tissues of untreated control mice. Intraperitoneal administration of LPS strongly induced SSeCKS mRNA expression in the lung, heart, liver, spleen, kidney, lymph node, adrenal gland, and pituitary gland, as well as in the brain. In lung and spleen, the SSeCKS mRNA levels increased almost 10-fold at 1 hr after LPS injection and persisted at high levels until 4 hr. Both in situ hybridization and immunohistochemical studies revealed that LPS administration conspicuously elevated expression of SSeCKS mRNA and protein in vascular endothelial cells of several organs. Ectopic expression of SSeCKS caused loss of cytoplasmic F-actin fibers in the mouse endothelial cell line LEII. These results indicate that SSeCKS is one of the major LPS-responsive proteins and may participate in alteration of cytoskeletal architecture in endothelial cells during inflammation.
|
| 巻・号 |
50(2)
|
| ページ |
245-55
|
| 公開日 |
2002-2-1
|
| DOI |
10.1177/002215540205000212
|
| PMID |
11799143
|
| MeSH |
A Kinase Anchor Proteins
Actins
Amino Acid Sequence
Animals
Blotting, Northern
Brain / blood supply
Cell Cycle Proteins*
Cells, Cultured
Endothelium, Vascular / metabolism*
Endothelium, Vascular / ultrastructure
Escherichia coli*
Immunohistochemistry
In Situ Hybridization
Lipopolysaccharides / pharmacology*
Mice
Mice, Inbred C57BL
Mitogens / biosynthesis*
Mitogens / genetics
Molecular Sequence Data
Muscle Fibers, Skeletal / metabolism
Organ Specificity
RNA, Messenger / biosynthesis
Rats
Rats, Wistar
|
| IF |
2.187
|
| リソース情報 |
| 遺伝子材料 |
pDEST12.2-SSECKS (RDB03015) |
