RRC ID 71883
Author Kitamura H, Okita K, Fujikura D, Mori K, Iwanaga T, Saito M.
Title Induction of Src-suppressed C kinase substrate (SSeCKS) in vascular endothelial cells by bacterial lipopolysaccharide.
Journal J Histochem Cytochem
Abstract We isolated cDNA of the mouse homologue of the src-suppressed C kinase substrate (SSeCKS) and analyzed the effects of lipopolysaccharide (LPS) injection on the tissue expression pattern of this protein. Northern blotting analysis showed that SSeCKS mRNA was expressed abundantly in the testis but at undetectable levels in other tissues of untreated control mice. Intraperitoneal administration of LPS strongly induced SSeCKS mRNA expression in the lung, heart, liver, spleen, kidney, lymph node, adrenal gland, and pituitary gland, as well as in the brain. In lung and spleen, the SSeCKS mRNA levels increased almost 10-fold at 1 hr after LPS injection and persisted at high levels until 4 hr. Both in situ hybridization and immunohistochemical studies revealed that LPS administration conspicuously elevated expression of SSeCKS mRNA and protein in vascular endothelial cells of several organs. Ectopic expression of SSeCKS caused loss of cytoplasmic F-actin fibers in the mouse endothelial cell line LEII. These results indicate that SSeCKS is one of the major LPS-responsive proteins and may participate in alteration of cytoskeletal architecture in endothelial cells during inflammation.
Volume 50(2)
Pages 245-55
Published 2002-2-1
DOI 10.1177/002215540205000212
PMID 11799143
MeSH A Kinase Anchor Proteins Actins Amino Acid Sequence Animals Blotting, Northern Brain / blood supply Cell Cycle Proteins* Cells, Cultured Endothelium, Vascular / metabolism* Endothelium, Vascular / ultrastructure Escherichia coli* Immunohistochemistry In Situ Hybridization Lipopolysaccharides / pharmacology* Mice Mice, Inbred C57BL Mitogens / biosynthesis* Mitogens / genetics Molecular Sequence Data Muscle Fibers, Skeletal / metabolism Organ Specificity RNA, Messenger / biosynthesis Rats Rats, Wistar
IF 2.187
DNA material pDEST12.2-SSECKS (RDB03015)