論文 - 詳細
| RRC ID | 72013 |
|---|---|
| 著者 | Ishikawa A, Waseda M, Ishii T, Kaneko MK, Kato Y, Kaneko S. |
| タイトル | Improved anti-solid tumor response by humanized anti-podoplanin chimeric antigen receptor transduced human cytotoxic T cells in an animal model. |
| ジャーナル | Genes Cells |
| Abstract |
Recently, research has been conducted with chimeric antigen receptor (CAR)-T cells to improve efficacy against solid tumors. Humanized CAR improved the long-term survival of CAR-T cells in patients' peripheral blood, resulting in increased therapeutic efficacy. Therefore, the humanization of the CAR-gene sequence is considered an effective method. Podoplanin (PDPN) is a glycosylated transmembrane protein that is highly expressed in solid tumors and is associated with poor prognosis in patients with cancer. Therefore, PDPN is considered a biomarker and good target for cancer treatment with CAR-T cells. Previously, an anti-PDPN CAR was generated from a conventional non-humanized antibody-NZ-1, the only anti-PDPN antibody for which a CAR was produced. In this study, we investigated other anti-PDPN CARs from the antibody NZ-27, or humanized NZ-1, to enhance the therapeutic potential of CAR-T cells. The CAR signal intensity was enhanced by the efficient expression of CAR proteins on the T-cell surface of NZ-27 CAR-T cells, which show tumor-specific cytotoxicity, proinflammatory cytokine production, and anti-tumor activity against PDPN-expressing tumor xenografts in mice that were significantly better than those in non-humanized NZ-1 CAR-T cells. This article is protected by copyright. All rights reserved. |
| 巻・号 | 27(9) |
| ページ | 549-558 |
| 公開日 | 2022-9-1 |
| DOI | 10.1111/gtc.12972 |
| PMID | 35790497 |
| MeSH | Animals Cell Line, Tumor Disease Models, Animal Humans Mice Neoplasms* / therapy Receptors, Chimeric Antigen* / genetics T-Lymphocytes* Xenograft Model Antitumor Assays |
| IF | 1.655 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MG-63(RCB1890) |