論文 - 詳細
| RRC ID | 72103 |
|---|---|
| 著者 | Miyoshi T, Friedman TB, Watanabe N. |
| タイトル | Fast-dissociating but highly specific antibodies are novel tools in biology, especially useful for multiplex super-resolution microscopy. |
| ジャーナル | STAR Protoc |
| Abstract |
Fast-dissociating, highly specific monoclonal antibodies (FDSAs) are single-molecule imaging probes useful for many biological assays including consecutive, multiplexable super-resolution microscopy. We developed a screening assay to characterize the kinetics of antibody-antigen interactions using single-molecule microscopy and established a pipeline to identify FDSAs from thousands of monoclonal candidates. Provided here are detailed protocols to prepare multi-well glass-bottom plates necessary for our assay to identify hybridoma clones secreting FDSAs. Synthesis of fluorescently labeled Fab fragments (Fab probes) from FDSAs is also described. For complete details on the use and execution of this protocol, please refer to Miyoshi et al. (2021). |
| 巻・号 | 2(4) |
| ページ | 100967 |
| 公開日 | 2021-12-17 |
| DOI | 10.1016/j.xpro.2021.100967 |
| PII | S2666-1667(21)00673-0 |
| PMID | 34841279 |
| PMC | PMC8605432 |
| MeSH | Animals Antibodies, Monoclonal* / chemistry Antibodies, Monoclonal* / metabolism Female Fluorescent Dyes* / chemistry Fluorescent Dyes* / metabolism Hybridomas / chemistry Hybridomas / metabolism Immunoglobulin Fab Fragments / chemistry Immunoglobulin Fab Fragments / metabolism Mice Mice, Inbred BALB C Microscopy, Fluorescence / methods* Single Molecule Imaging / methods* |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | UV-P3U1-B8(RCB1157) |