論文 - 詳細
| RRC ID | 72212 |
|---|---|
| 著者 | Nittayaboon K, Leetanaporn K, Sangkhathat S, Roytrakul S, Navakanitworakul R. |
| タイトル | Cytotoxic effect of metformin on butyrate-resistant PMF-K014 colorectal cancer spheroid cells. |
| ジャーナル | Biomed Pharmacother |
| Abstract |
Three-dimensional (3D) cell culture models are used in cancer research because they mimic physiological responses in vivo compared with two-dimensional (2D) culture systems. Recently, cross-resistance of butyrate-resistant (BR) cells and chemoresistance in colorectal cancer (CRC) cells have been reported; however, effective treatments for BR cells have not been identified. In this study, we investigated the cytotoxicity of metformin (MET), an anti-diabetic drug, on BR CRC cells in a 3D spheroid culture model. The results demonstrate that MET decreases spheroid size, migration, and spheroid viability, while it increases spheroid death. The molecular mechanism revealed that AMP-activated protein kinase (AMPK) and Akt serine/threonine kinase 1(Akt) were significantly upregulated, whereas the acetyl-CoA-carboxylase (ACC) and mammalian target of rapamycin (mTOR) were downregulated, which led to caspase activation and apoptosis. Our findings show the potential cytotoxicity of MET on CRC-BR cells. The combination of MET and conventional chemotherapeutic drugs should be addressed in further studies to reduce the side effects of standard chemotherapy for CRC. |
| 巻・号 | 151 |
| ページ | 113214 |
| 公開日 | 2022-7-1 |
| DOI | 10.1016/j.biopha.2022.113214 |
| PII | S0753-3322(22)00603-5 |
| PMID | 35676792 |
| MeSH | AMP-Activated Protein Kinases / metabolism Antineoplastic Agents* / pharmacology Antineoplastic Agents* / therapeutic use Butyrates / pharmacology Butyrates / therapeutic use Cell Line, Tumor Colorectal Neoplasms* / drug therapy Humans Metformin* / pharmacology Metformin* / therapeutic use Proto-Oncogene Proteins c-akt / metabolism |
| IF | 4.545 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | PMF-ko14(RCB1426) |