論文 - 詳細
| RRC ID | 72243 |
|---|---|
| 著者 | Fujimoto M, Takii R, Matsumoto M, Okada M, Nakayama KI, Nakato R, Fujiki K, Shirahige K, Nakai A. |
| タイトル | HSF1 phosphorylation establishes an active chromatin state via the TRRAP-TIP60 complex and promotes tumorigenesis. |
| ジャーナル | Nat Commun |
| Abstract |
Transcriptional regulation by RNA polymerase II is associated with changes in chromatin structure. Activated and promoter-bound heat shock transcription factor 1 (HSF1) recruits transcriptional co-activators, including histone-modifying enzymes; however, the mechanisms underlying chromatin opening remain unclear. Here, we demonstrate that HSF1 recruits the TRRAP-TIP60 acetyltransferase complex in HSP72 promoter during heat shock in a manner dependent on phosphorylation of HSF1-S419. TRIM33, a bromodomain-containing ubiquitin ligase, is then recruited to the promoter by interactions with HSF1 and a TIP60-mediated acetylation mark, and cooperates with the related factor TRIM24 for mono-ubiquitination of histone H2B on K120. These changes in histone modifications are triggered by phosphorylation of HSF1-S419 via PLK1, and stabilize the HSF1-transcription complex in HSP72 promoter. Furthermore, HSF1-S419 phosphorylation is constitutively enhanced in and promotes proliferation of melanoma cells. Our results provide mechanisms for HSF1 phosphorylation-dependent establishment of an active chromatin status, which is important for tumorigenesis. |
| 巻・号 | 13(1) |
| ページ | 4355 |
| 公開日 | 2022-7-29 |
| DOI | 10.1038/s41467-022-32034-4 |
| PII | 10.1038/s41467-022-32034-4 |
| PMID | 35906200 |
| PMC | PMC9338313 |
| MeSH | Adaptor Proteins, Signal Transducing / metabolism Carcinogenesis / genetics Chromatin* Heat Shock Transcription Factors / genetics Heat Shock Transcription Factors / metabolism Histones* / metabolism Humans Lysine Acetyltransferase 5 / metabolism Nuclear Proteins / metabolism Phosphorylation Protein Binding Transcription Factors / genetics |
| IF | 12.121 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MMAc(RCB0808) MCF7(RCB1904) Hep G2(RCB1648) HCT116(RCB2979) A549(RCB0098) LNCap.FGC(RCB2144) PANC-1(RCB2095) |