| RRC ID |
72298
|
| Author |
Sun W, Hattori N, Mutai H, Toyoshima Y, Kimura H, Tanaka S, Shiota K.
|
| Title |
PAL31, a nuclear protein required for progression to the S phase.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
PAL31 is a nuclear protein expressed by various cell types. In the present study, the expression and function of PAL31 were examined in the cytokine-regulated growth of T and B cell lines. Treatment of the cells with mitogens [ovine PRL, recombinant rat placental lactogen-I (PL-I) and human IL-3] caused a dose-dependent increase in the expression of PAL31 mRNA in the PRL-dependent cell line Nb2, and IL-3 dependent cell line BaF3. A time-course study on synchronized Nb2 cells revealed that the expression of PAL31 is specific to the late G1 and S phases. Immunocytological studies revealed that PAL31 accumulates in the nuclei at the S phase. Furthermore, the antisense oligonucleotide for PAL31 severely inhibited the proliferation of Nb2 cells by inhibiting cells progressing to the S phase. Thus, PAL31 is a nuclear protein associated with cell cycle progression.
|
| Volume |
280(4)
|
| Pages |
1048-54
|
| Published |
2001-2-2
|
| DOI |
10.1006/bbrc.2000.4244
|
| PII |
S0006-291X(00)94244-X
|
| PMID |
11162633
|
| MeSH |
Animals
B-Lymphocytes / metabolism*
Blotting, Western
Cell Cycle
Cell Cycle Proteins*
Cell Division
Cell Line
Dose-Response Relationship, Drug
Flow Cytometry
Fluorescent Antibody Technique
G1 Phase
Humans
Immunohistochemistry
Interleukin-3 / pharmacology
Molecular Chaperones
Nerve Tissue Proteins*
Nuclear Proteins / biosynthesis*
Nuclear Proteins / physiology*
Oligonucleotides, Antisense / metabolism
Placental Lactogen / pharmacology
Prolactin / pharmacology
RNA, Messenger / metabolism
Rats
S Phase*
Sheep
T-Lymphocytes / metabolism*
Time Factors
Tumor Cells, Cultured
|
| IF |
2.985
|
| Resource |
| Human and Animal Cells |
Ba/F3(RCB0805)
WEHI -3(RCB0035) |
