論文 - 詳細
| RRC ID | 72298 |
|---|---|
| 著者 | Sun W, Hattori N, Mutai H, Toyoshima Y, Kimura H, Tanaka S, Shiota K. |
| タイトル | PAL31, a nuclear protein required for progression to the S phase. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
PAL31 is a nuclear protein expressed by various cell types. In the present study, the expression and function of PAL31 were examined in the cytokine-regulated growth of T and B cell lines. Treatment of the cells with mitogens [ovine PRL, recombinant rat placental lactogen-I (PL-I) and human IL-3] caused a dose-dependent increase in the expression of PAL31 mRNA in the PRL-dependent cell line Nb2, and IL-3 dependent cell line BaF3. A time-course study on synchronized Nb2 cells revealed that the expression of PAL31 is specific to the late G1 and S phases. Immunocytological studies revealed that PAL31 accumulates in the nuclei at the S phase. Furthermore, the antisense oligonucleotide for PAL31 severely inhibited the proliferation of Nb2 cells by inhibiting cells progressing to the S phase. Thus, PAL31 is a nuclear protein associated with cell cycle progression. |
| 巻・号 | 280(4) |
| ページ | 1048-54 |
| 公開日 | 2001-2-2 |
| DOI | 10.1006/bbrc.2000.4244 |
| PII | S0006-291X(00)94244-X |
| PMID | 11162633 |
| MeSH | Animals B-Lymphocytes / metabolism* Blotting, Western Cell Cycle Cell Cycle Proteins* Cell Division Cell Line Dose-Response Relationship, Drug Flow Cytometry Fluorescent Antibody Technique G1 Phase Humans Immunohistochemistry Interleukin-3 / pharmacology Molecular Chaperones Nerve Tissue Proteins* Nuclear Proteins / biosynthesis* Nuclear Proteins / physiology* Oligonucleotides, Antisense / metabolism Placental Lactogen / pharmacology Prolactin / pharmacology RNA, Messenger / metabolism Rats S Phase* Sheep T-Lymphocytes / metabolism* Time Factors Tumor Cells, Cultured |
| IF | 2.985 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | Ba/F3(RCB0805) WEHI -3(RCB0035) |