RRC ID 723
Author Cho KH, Shindo T, Kim GT, Nitasaka E, Tsukaya H.
Title Characterization of a member of the AN subfamily, IAN, from Ipomoea nil.
Journal Plant Cell Physiol.
Abstract ANGUSTIFOLIA (AN) is the first C-terminal binding protein (CtBP) gene from plants and controls leaf width and pattern of trichome branching in Arabidopsis thaliana (L.) Heynh. We characterized an ortholog of AN from Ipomoea nil (L.) Roth (Japanese morning glory) and designated it Ipomoea nil's AN (IAN). IAN is a single-copy gene in the genome and is expressed ubiquitously in various organs of I. nil. IAN contains not only a D2-HDH motif, which is highly conserved within the CtBP family, but also LXCXE, NLS and PEST motifs, which are specific to the AN subfamily. The expression of IAN cDNA driven by the cauliflower mosaic virus 35S promoter restored a defect in leaf expansion in the leaf width direction in the angustifolia-1 (an-1) mutant of Arabidopsis, suggesting that IAN retains a common function with AN. In contrast, the complementation by IAN of a defect in the trichome branching pattern on the leaf surface of the an-1 mutant was less effective than that observed for leaf shape. These results suggest that the mechanisms by which AN regulates leaf width and trichome branching are separable.
Volume 46(1)
Pages 250-5
Published 2005-1
DOI 10.1093/pcp/pci020
PII pci020
PMID 15659438
MeSH Amino Acid Sequence Arabidopsis / genetics Arabidopsis Proteins / genetics Base Sequence DNA, Complementary / genetics DNA, Plant / genetics Genes, Plant* Ipomoea / genetics* Ipomoea / growth & development Ipomoea / metabolism Molecular Sequence Data Mutation Plants, Genetically Modified RNA, Messenger / genetics RNA, Messenger / metabolism RNA, Plant / genetics RNA, Plant / metabolism Repressor Proteins / genetics Sequence Homology, Amino Acid Species Specificity
IF 4.059
Times Cited 13
Morning Glory AK1