| RRC ID |
72311
|
| 著者 |
Fu L, Zhang D, Yi N, Cao Y, Wei Y, Wang W, Li L.
|
| タイトル |
Circular RNA circPBX3 promotes cisplatin resistance of ovarian cancer cells via interacting with IGF2BP2 to stabilize ATP7A mRNA expression.
|
| ジャーナル |
Hum Cell
|
| Abstract |
Circular RNAs (circRNAs) are a class of non-coding RNAs with a unique covalently closed loop structure. Recent studies indicate that dysregulation of circRNAs acts a role in cancer progression and chemotherapy resistance via interacting with RNA-binding proteins (RBPs). Herein, we identified circPBX3 to be involved in cisplatin resistance of ovarian cancer. In our study, two cisplatin-resistant ovarian cancer cell lines were established, and transcriptome RNA-sequencing was performed and circPBX3 was identified as significantly upregulated circRNA in these cells. The characteristics of circPBX3 and potential function of circPBX3 were evaluated. We found that circPBX3 was upregulated in ovarian tumor tissues and cisplatin-resistant ovarian cancer cells. CircPBX3 overexpression increased the half maximal inhibitory rate (IC50) of cisplatin, promoted colony formation and tumor xenografts growth, and reduced cell apoptosis of ovarian cancer cells under cisplatin treatment, while silencing circPBX3 showed opposite effects. Furthermore, circPBX3 could interact with the RNA-binding protein IGF2BP2, thus increased the stability of ATP7A mRNA and elevated ATP7A protein level. In addition, silencing ATP7A in ovarian cancer cells abrogated the effect of circPBX3 overexpression on cisplatin tolerance. Our findings provided a novel role of circPBX3 in cisplatin resistance of ovarian cancer.
|
| 巻・号 |
35(5)
|
| ページ |
1560-1576
|
| 公開日 |
2022-9-1
|
| DOI |
10.1007/s13577-022-00748-8
|
| PII |
10.1007/s13577-022-00748-8
|
| PMID |
35907138
|
| MeSH |
Antineoplastic Agents* / pharmacology
Antineoplastic Agents* / therapeutic use
Cell Line, Tumor
Cisplatin / pharmacology
Cisplatin / therapeutic use
Copper-Transporting ATPases / genetics
Copper-Transporting ATPases / metabolism
Drug Resistance, Neoplasm / genetics
Female
Gene Expression Regulation, Neoplastic / genetics
Humans
Ovarian Neoplasms* / drug therapy
Ovarian Neoplasms* / genetics
Ovarian Neoplasms* / pathology
Peptide Fragments / genetics
Peptide Fragments / metabolism
Peptide Fragments / pharmacology
RNA, Circular / genetics
RNA, Messenger
RNA-Binding Proteins / genetics
RNA-Binding Proteins / metabolism
|
| IF |
3.463
|
| リソース情報 |
| ヒト・動物細胞 |
JHOS-2(RCB1521)
JHOS-4(RCB1678) |
