論文 - 詳細
| RRC ID | 72325 |
|---|---|
| 著者 | Nakazato T, Kawamura T, Uemura T, Liu L, Li J, Sasai M, Harada A, Ito E, Iseoka H, Toda K, Sawa Y, Miyagawa S. |
| タイトル | Engineered three-dimensional cardiac tissues maturing in a rotating wall vessel bioreactor remodel diseased hearts in rats with myocardial infarction. |
| ジャーナル | Stem Cell Reports |
| Abstract |
A rotating wall vessel (RWV) bioreactor was constructed for growing massive functional cardiac constructs to recover the function of a distressed rat heart. Three-dimensional cardiac tissues were engineered by seeding human-induced pluripotent stem cell-derived cardiomyocytes on poly(lactic-co-glycolic acid) fiber sheets (3D-hiPSC-CTs) and cultured in the RWV bioreactor (RWV group) or under static conditions (control group). The tissues were transplanted into a myocardial infarction nude rat model, and cardiac performance was evaluated. In the RWV group, cell viability and contractile and electrical properties significantly improved, mature cardiomyocytes were observed, and mechanical stress-related mediators of mammalian target of rapamycin signaling were upregulated compared with those of the control. Four weeks post-transplantation, tissue survival and left ventricular ejection fraction significantly improved in the RWV group. Hence, dynamic culture in an RWV bioreactor could provide a superior culture environment for improved performance of 3D-hiPSC-CTs, providing a means for functional cardiomyogenesis in myocyte-loss heart failure. |
| 巻・号 | 17(5) |
| ページ | 1170-1182 |
| 公開日 | 2022-5-10 |
| DOI | 10.1016/j.stemcr.2022.03.012 |
| PII | S2213-6711(22)00145-X |
| PMID | 35427484 |
| PMC | PMC9133656 |
| MeSH | Animals Bioreactors Mammals Myocardial Infarction* / therapy Myocytes, Cardiac / transplantation Rats Rats, Nude Stroke Volume Tissue Engineering / methods Ventricular Function, Left* |
| IF | 6.032 |
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| 最多言及媒体 | Blogs |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 253G1(HPS0002) |