RRC ID 72328
Author Banerjee K, Madhyastha H, Sandur V R, N T M, N T, Thiagarajan P.
Title Anti-inflammatory and wound healing potential of a clove oil emulsion.
Journal Colloids Surf B Biointerfaces
Abstract Emulsion formulations of essential oils are of major interest due to their relative biosafety, biocompatibility and good pharmacological potential. Their structural constituents (oil and water phase) facilitate ready solubilization of incorporated hydrophilic/lipophilic actives for their targeted delivery. In the present study, m5S cells were tested for their viability at various concentrations of clove oil and an alkyl polyglucoside emulsifier, viz., Montanov 202™. Thereafter, good cell viable concentrations of oil (10 %) and emulsifier (4%) were used at their optimised ratio (1:0.4) to formulate an oil in water emulsion using phase inversion technique followed by ultrasonication for particle size reduction. Gas chromatography-mass spectrometry (GC-MS) analysis of clove oil revealed eugenol (76.11 %) and eugenyl acetate (12.41 %) as major constituents. The formulated clove oil emulsion was then characterised with respect to its size, zeta potential, microscopic and thermal analysis and the presence of liquid crystals were observed in the same. It was further studied for its anti-inflammatory potential in female Wistar rats wherein topical treatment with the emulsion inhibited paw swelling induced by carrageenan model by 40-60% over 30-180 min compared to untreated animals. Similarly, the emulsion's wound healing potential was also significant with respect to wounds induced by both incision (wound breaking strength of 338.91 ± 5.02 g) and excision (95 % wound contraction by 16th day) model in these animals, with a re-epithelization period of 10.67 ± 1.67 days and results being comparable with diclofenac gel and neomycin cream (positive controls). Histopathology of the skin sections showed accelerated healing with early granular tissue and collagen formation in emulsion treated animals. It is hence envisaged that this clove oil emulsion can substitute chemical based topical products for anti-inflammatory and wound healing applications due to its biological constituents as well as because of the presence of liquid crystals in its formulation.
Volume 193
Pages 111102
Published 2020-9-1
DOI 10.1016/j.colsurfb.2020.111102
PII S0927-7765(20)30332-5
PMID 32442923
MeSH Animals Anti-Inflammatory Agents, Non-Steroidal / administration & dosage Anti-Inflammatory Agents, Non-Steroidal / chemistry Anti-Inflammatory Agents, Non-Steroidal / pharmacology* Carrageenan Cell Proliferation / drug effects Cell Survival / drug effects Cells, Cultured Clove Oil / administration & dosage Clove Oil / chemistry Clove Oil / pharmacology Edema / chemically induced Edema / drug therapy* Emulsions / chemistry Emulsions / pharmacology Female Mice Oxidative Stress / drug effects Particle Size Rats Rats, Wistar Surface Properties Wound Healing / drug effects*
IF 4.389
Human and Animal Cells MS-5(RCB4680)