| RRC ID |
72352
|
| 著者 |
Noguchi T, Ikeda K, Yamamoto K, Yoshida I, Ashiba A, Tsuchiyama J, Shinagawa K, Yoshino T, Takata M, Harada M.
|
| タイトル |
Antisense oligodeoxynucleotides to latent membrane protein 1 induce growth inhibition, apoptosis and Bcl-2 suppression in Epstein-Barr virus (EBV)-transformed B-lymphoblastoid cells, but not in EBV-positive natural killer cell lymphoma cells.
|
| ジャーナル |
Br J Haematol
|
| Abstract |
Epstein-Barr virus (EBV)-encoded latent membrane protein 1 (LMP-1) is essential for immortalization of B cells by EBV, protects the infected cells from apoptotic cell death and induces Bcl-2 expression. Suppression of LMP-1 expression by antisense oligodeoxynucleotides (AS-oligo) to LMP-1 inhibits proliferation, promotes apoptosis and suppresses Bcl-2 expression in EBV-transformed B cells. However, the function of LMP-1 expression in EBV-positive natural killer (NK) cell lymphoma cells has not been reported previously. We examined the function of LMP-1 in two EBV-positive NK cell lymphoma cell lines (NK-YS and YT) through suppressing LMP-1 expression by AS-oligo to LMP-1. The AS-oligo to LMP-1 suppressed LMP-1 mRNA and protein expression in two EBV-positive NK cell lymphoma cell lines, as well as in an EBV-transformed B-cell line (CMG-1). Proliferation was inhibited, apoptosis was induced and Bcl-2 expression was suppressed in CMG-1 cells, but none of these events were observed in NK-YS or YT cells. These results suggest that proliferation, inhibition of apoptosis and Bcl-2 expression in EBV-positive NK cell lymphoma cells are not directly regulated by LMP-1 as in EBV-transformed B-cell lines, but are probably mediated through other signal transducing systems.
|
| 巻・号 |
114(1)
|
| ページ |
84-92
|
| 公開日 |
2001-7-1
|
| DOI |
10.1046/j.1365-2141.2001.02887.x
|
| PII |
bjh2887
|
| PMID |
11472349
|
| MeSH |
Adaptor Proteins, Signal Transducing
Apoptosis / drug effects
Blotting, Western
Carrier Proteins / genetics*
Cell Division / drug effects
Cell Line, Transformed
Cytoskeletal Proteins
Gene Deletion
Gene Expression / drug effects
Herpesvirus 4, Human*
Humans
Intracellular Signaling Peptides and Proteins
Killer Cells, Natural / metabolism
Killer Cells, Natural / virology*
LIM Domain Proteins
Lymphoma, T-Cell / metabolism
Lymphoma, T-Cell / virology*
Oligonucleotides, Antisense / pharmacology*
Proto-Oncogene Proteins c-bcl-2 / genetics
Reverse Transcriptase Polymerase Chain Reaction
Tumor Cells, Cultured
|
| IF |
5.518
|
| リソース情報 |
| ヒト・動物細胞 |
CGM1(RCB0566) |
