RRC ID 72352
Author Noguchi T, Ikeda K, Yamamoto K, Yoshida I, Ashiba A, Tsuchiyama J, Shinagawa K, Yoshino T, Takata M, Harada M.
Title Antisense oligodeoxynucleotides to latent membrane protein 1 induce growth inhibition, apoptosis and Bcl-2 suppression in Epstein-Barr virus (EBV)-transformed B-lymphoblastoid cells, but not in EBV-positive natural killer cell lymphoma cells.
Journal Br J Haematol
Abstract Epstein-Barr virus (EBV)-encoded latent membrane protein 1 (LMP-1) is essential for immortalization of B cells by EBV, protects the infected cells from apoptotic cell death and induces Bcl-2 expression. Suppression of LMP-1 expression by antisense oligodeoxynucleotides (AS-oligo) to LMP-1 inhibits proliferation, promotes apoptosis and suppresses Bcl-2 expression in EBV-transformed B cells. However, the function of LMP-1 expression in EBV-positive natural killer (NK) cell lymphoma cells has not been reported previously. We examined the function of LMP-1 in two EBV-positive NK cell lymphoma cell lines (NK-YS and YT) through suppressing LMP-1 expression by AS-oligo to LMP-1. The AS-oligo to LMP-1 suppressed LMP-1 mRNA and protein expression in two EBV-positive NK cell lymphoma cell lines, as well as in an EBV-transformed B-cell line (CMG-1). Proliferation was inhibited, apoptosis was induced and Bcl-2 expression was suppressed in CMG-1 cells, but none of these events were observed in NK-YS or YT cells. These results suggest that proliferation, inhibition of apoptosis and Bcl-2 expression in EBV-positive NK cell lymphoma cells are not directly regulated by LMP-1 as in EBV-transformed B-cell lines, but are probably mediated through other signal transducing systems.
Volume 114(1)
Pages 84-92
Published 2001-7-1
DOI 10.1046/j.1365-2141.2001.02887.x
PII bjh2887
PMID 11472349
MeSH Adaptor Proteins, Signal Transducing Apoptosis / drug effects Blotting, Western Carrier Proteins / genetics* Cell Division / drug effects Cell Line, Transformed Cytoskeletal Proteins Gene Deletion Gene Expression / drug effects Herpesvirus 4, Human* Humans Intracellular Signaling Peptides and Proteins Killer Cells, Natural / metabolism Killer Cells, Natural / virology* LIM Domain Proteins Lymphoma, T-Cell / metabolism Lymphoma, T-Cell / virology* Oligonucleotides, Antisense / pharmacology* Proto-Oncogene Proteins c-bcl-2 / genetics Reverse Transcriptase Polymerase Chain Reaction Tumor Cells, Cultured
IF 5.518
Human and Animal Cells CGM1(RCB0566)