RRC ID 72354
Author Sakamoto M, Ota K, Kondo Y, Okamura M, Fujii H, Bito H.
Title In utero electroporation and cranial window implantation for in vivo wide-field two-photon calcium imaging using G-CaMP9a transgenic mice.
Journal STAR Protoc
Abstract We present a protocol to prepare mouse cranial window implantation for in vivo two-photon wide-field calcium imaging. This protocol uses G-CaMP9a transgenic mice, which express a genetically encoded calcium indicator with high signal-to-noise ratio. We describe in utero electroporation, followed by headplate fixation and cranial window implantation. This protocol can be used for measuring neural activity and is suitable for long-term imaging in large populations. Moreover, this protocol does not require preparation of Flp-expressing transgenic mice. For complete details on the use and execution of this protocol, please refer to Sakamoto et al. (2022).
Volume 3(2)
Pages 101421
Published 2022-6-17
DOI 10.1016/j.xpro.2022.101421
PII S2666-1667(22)00301-X
PMID 35693213
PMC PMC9185017
MeSH Animals Calcium, Dietary Diagnostic Imaging Electroporation* Mice Mice, Transgenic Skull* / surgery
DNA material pCAG-Flpo (RDB19603) pCAG-mCherry (RDB19604)