| RRC ID |
72396
|
| 著者 |
Deai M, Oya R, Saso N, Tanaka A, Uchida I, Miyake Y, Tachihara R, Otsugu M, Mine A, Sato K, Tomura H.
|
| タイトル |
Ethylenediaminetetraacetic acid (EDTA) enhances cAMP production in human TDAG8-expressing cells.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
Ethylenediaminetetraacetic acid (EDTA) is a chelating agent that binds tightly to metal ions. We found that cAMP response element (CRE)-driven promoter activity by protons was enhanced by EDTA in human T-cell death-associated gene 8 (TDAG8)-overexpressed HEK293T cells. The enhancing action by EDTA was also detected by proton-induced cAMP production that is located upstream from the CRE-driven promoter activity even at physiological proton concentration pH7.4. The proton-induced CRE-driven promoter activity was not enhanced by other chelating agents, ethylene glycol tetraacetic acid (EGTA) and sodium citrate. The enhanced CRE-driven promoter activity by EDTA was not attenuated by increasing the extracellular calcium ion concentration. These results indicate that the EDTA-enhancing action may not be due to its chelating action but might rather be another EDTA-specific effect. Enhanced cAMP production by EDTA was also detected in a human leukemia cell line HL-60, in which TDAG8 and OGR1 (ovarian cancer G-protein-coupled receptor 1) were endogenously expressed, suggesting that the medical use of EDTA would influence the physiological and pathophysiological functions of hematopoietic cells.
|
| 巻・号 |
626
|
| ページ |
15-20
|
| 公開日 |
2022-10-20
|
| DOI |
10.1016/j.bbrc.2022.07.110
|
| PII |
S0006-291X(22)01096-8
|
| PMID |
35964552
|
| MeSH |
Cyclic AMP* / metabolism
Edetic Acid / pharmacology
HEK293 Cells
Humans
Hydrogen-Ion Concentration
Protons*
|
| IF |
2.985
|
| リソース情報 |
| ヒト・動物細胞 |
293T(RCB2202) |
