論文 - 詳細
| RRC ID | 72396 |
|---|---|
| 著者 | Deai M, Oya R, Saso N, Tanaka A, Uchida I, Miyake Y, Tachihara R, Otsugu M, Mine A, Sato K, Tomura H. |
| タイトル | Ethylenediaminetetraacetic acid (EDTA) enhances cAMP production in human TDAG8-expressing cells. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
Ethylenediaminetetraacetic acid (EDTA) is a chelating agent that binds tightly to metal ions. We found that cAMP response element (CRE)-driven promoter activity by protons was enhanced by EDTA in human T-cell death-associated gene 8 (TDAG8)-overexpressed HEK293T cells. The enhancing action by EDTA was also detected by proton-induced cAMP production that is located upstream from the CRE-driven promoter activity even at physiological proton concentration pH7.4. The proton-induced CRE-driven promoter activity was not enhanced by other chelating agents, ethylene glycol tetraacetic acid (EGTA) and sodium citrate. The enhanced CRE-driven promoter activity by EDTA was not attenuated by increasing the extracellular calcium ion concentration. These results indicate that the EDTA-enhancing action may not be due to its chelating action but might rather be another EDTA-specific effect. Enhanced cAMP production by EDTA was also detected in a human leukemia cell line HL-60, in which TDAG8 and OGR1 (ovarian cancer G-protein-coupled receptor 1) were endogenously expressed, suggesting that the medical use of EDTA would influence the physiological and pathophysiological functions of hematopoietic cells. |
| 巻・号 | 626 |
| ページ | 15-20 |
| 公開日 | 2022-10-20 |
| DOI | 10.1016/j.bbrc.2022.07.110 |
| PII | S0006-291X(22)01096-8 |
| PMID | 35964552 |
| MeSH | Cyclic AMP* / metabolism Edetic Acid / pharmacology HEK293 Cells Humans Hydrogen-Ion Concentration Protons* |
| IF | 2.985 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 293T(RCB2202) |