論文 - 詳細
| RRC ID | 72414 |
|---|---|
| 著者 | Blessing C, Apelt K, van den Heuvel D, Gonzalez-Leal C, Rother MB, van der Woude M, González-Prieto R, Yifrach A, Parnas A, Shah RG, Kuo TT, Boer DEC, Cai J, Kragten A, Kim HS, Schärer OD, Vertegaal ACO, Shah GM, Adar S, Lans H, van Attikum H, Ladurner AG, Luijsterburg MS. |
| タイトル | XPC-PARP complexes engage the chromatin remodeler ALC1 to catalyze global genome DNA damage repair. |
| ジャーナル | Nat Commun |
| Abstract |
Cells employ global genome nucleotide excision repair (GGR) to eliminate a broad spectrum of DNA lesions, including those induced by UV light. The lesion-recognition factor XPC initiates repair of helix-destabilizing DNA lesions, but binds poorly to lesions such as CPDs that do not destabilize DNA. How difficult-to-repair lesions are detected in chromatin is unknown. Here, we identify the poly-(ADP-ribose) polymerases PARP1 and PARP2 as constitutive interactors of XPC. Their interaction results in the XPC-stimulated synthesis of poly-(ADP-ribose) (PAR) by PARP1 at UV lesions, which in turn enables the recruitment and activation of the PAR-regulated chromatin remodeler ALC1. PARP2, on the other hand, modulates the retention of ALC1 at DNA damage sites. Notably, ALC1 mediates chromatin expansion at UV-induced DNA lesions, leading to the timely clearing of CPD lesions. Thus, we reveal how chromatin containing difficult-to-repair DNA lesions is primed for repair, providing insight into mechanisms of chromatin plasticity during GGR. |
| 巻・号 | 13(1) |
| ページ | 4762 |
| 公開日 | 2022-8-13 |
| DOI | 10.1038/s41467-022-31820-4 |
| PII | 10.1038/s41467-022-31820-4 |
| PMID | 35963869 |
| PMC | PMC9376112 |
| MeSH | Chromatin* / genetics DNA / genetics DNA / metabolism DNA Damage DNA Repair DNA-Binding Proteins / metabolism Poly Adenosine Diphosphate Ribose / metabolism Poly(ADP-ribose) Polymerase Inhibitors* |
| IF | 12.121 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 20 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 10H(RCB0705) |