Reference - Detail
| RRC ID | 72453 |
|---|---|
| Author | Tsukano K, Yamamoto T, Watanabe T, Michiue T. |
| Title | Xenopus Dusp6 modulates FGF signaling to precisely pattern pre-placodal ectoderm. |
| Journal | Dev Biol |
| Abstract |
Pre-placodal ectoderm (PPE), a horseshoe-shaped narrow region formed during early vertebrate development, gives rise to multiple types of sensory organs and ganglia. For PPE induction, a certain level of FGF signal activation is required. However, it is difficult to reproducibly induce the narrow region with variations in gene expression, including FGF, among individuals. An intracellular regulatory factor of FGF signaling, Dusp6, is expressed by FGF signal activation and inactivates a downstream regulator, ERK1/2, in adult tissues; however, its role in early development is not well known. Here, we reveal that Dusp6 is expressed in an FGF-dependent manner in Xenopus PPE. Gain- and loss-of-function experiments showed that Dusp6 is required for expression of a PPE gene, Six1, and patterning of adjacent regions, neural plate, and neural crest. To reveal the importance of Dusp6 in variable FGF production, we performed Dusp6 knockdown with FGF-bead implantation, which resulted in varying Six1 expression patterns. Taken together, these results suggest that Dusp6 is required for PPE formation and that it contributes to the robust patterning of PPE by mediating FGF signaling. |
| Volume | 488 |
| Pages | 81-90 |
| Published | 2022-8-1 |
| DOI | 10.1016/j.ydbio.2022.05.009 |
| PII | S0012-1606(22)00097-5 |
| PMID | 35598626 |
| MeSH | Animals Dual Specificity Phosphatase 6 / genetics Dual Specificity Phosphatase 6 / metabolism Ectoderm* / metabolism Gene Expression Regulation, Developmental / genetics Homeodomain Proteins / metabolism Humans Neural Crest / metabolism Neural Plate* / metabolism Xenopus Proteins / genetics Xenopus Proteins / metabolism Xenopus laevis / genetics Xenopus laevis / metabolism |
| IF | 2.896 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 17 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Clawed frogs / Newts | Xenopus tropicalis |