RRC ID 72454
Author Ji R, Jia FY, Chen X, Wang ZH, Jin WY, Yang J.
Title Salidroside alleviates oxidative stress and apoptosis via AMPK/Nrf2 pathway in DHT-induced human granulosa cell line KGN.
Journal Arch Biochem Biophys
Abstract In the past few years, emerging evidence established persistent oxidative stress to be a key player in the pathogenesis of polycystic ovary syndrome (PCOS). Particularly, it damages the function of granulosa cells, and thus hinders the development of follicles. The present study aimed to explore and establish the protective effects of salidroside on dihydrotestosterone (DHT)-induced Granulosa-like tumor cell line (KGN), mediated via antioxidant mechanisms. The study assessed the positive effects of salidroside on DHT-induced apoptosis, reactive oxygen species (ROS) accumulation, damage of antioxidant capacity, and mitochondrial membrane potential depolarization. Interestingly, salidroside partly reversed DHT mediated effects, via stimulation of nuclear factor erythroid 2-related factor 2 (Nrf2) signaling pathway and the downstream antioxidant proteins heme oxygenase-1(HO-1) and quinine oxidoreductase 1(NQO1). Additionally, the knockdown of Nrf2 partly moderated the antioxidant and anti-apoptosis effects of salidroside in DHT-treated KGN cells. Mechanistically, AMP-activated protein kinase (AMPK) was identified to be the upstream signaling involved in salidroside-induced Nrf2 activation, as silencing of AMPK partly prevented the upregulation of Nrf2 and the downstream proteins HO-1 and NQO1. Altogether, the present study is the first to effectively demonstrate the inhibitory effect of salidroside on DHT-stimulated oxidative stress and apoptosis in KGN cells, which was dependent on Nrf2 activation that involved AMPK.
Volume 715
Pages 109094
Published 2022-1-15
DOI 10.1016/j.abb.2021.109094
PII S0003-9861(21)00343-X
PMID 34813774
MeSH AMP-Activated Protein Kinases / metabolism Antioxidants / pharmacology* Apoptosis / drug effects* Cell Line, Tumor Cell Nucleus / metabolism Dihydrotestosterone / pharmacology* Gene Knockdown Techniques Glucosides / pharmacology* Humans NF-E2-Related Factor 2 / genetics NF-E2-Related Factor 2 / metabolism Oxidative Stress / drug effects* Phenols / pharmacology* Signal Transduction / drug effects*
IF 3.343
Human and Animal Cells KGN(RCB1154)