RRC ID 72479
著者 Bouazzi L, Sproll P, Eid W, Biason-Lauber A.
タイトル The transcriptional regulator CBX2 and ovarian function: A whole genome and whole transcriptome approach.
ジャーナル Sci Rep
Abstract The chromobox homolog 2 (CBX2) was found to be important for human testis development, but its role in the human ovary remains elusive. We conducted a genome-wide analysis based on DNA adenine methyltransferase identification (DamID) and RNA sequencing strategies to investigate CBX2 in the human granulosa cells. Functional analysis revealed that CBX2 was upstream of genes contributing to ovarian function like folliculogenesis and steroidogenesis (i.e. ESR1, NRG1, AKR1C1, PTGER2, BMP15, BMP2, FSHR and NTRK1/2). We identified CBX2 regulated genes associated with polycystic ovary syndrome (PCOS) such as TGFβ, MAP3K15 and DKK1, as well as genes implicated in premature ovarian failure (POF) (i.e. POF1B, BMP15 and HOXA13) and the pituitary deficiency (i.e. LHX4 and KISS1). Our study provided an excellent opportunity to identify genes surrounding CBX2 in the ovary and might contribute to the understanding of ovarian physiopathology causing infertility in women.
巻・号 9(1)
ページ 17033
公開日 2019-11-19
DOI 10.1038/s41598-019-53370-4
PII 10.1038/s41598-019-53370-4
PMID 31745224
PMC PMC6864077
MeSH Cell Line, Tumor Female Gene Expression Regulation, Developmental / genetics* Genome, Human / genetics Granulosa Cells / cytology* Humans Infertility, Female / genetics Middle Aged Ovary / embryology* Pituitary Diseases / genetics Polycomb Repressive Complex 1 / genetics Polycomb Repressive Complex 1 / metabolism* Polycystic Ovary Syndrome / genetics Protein Isoforms / genetics Transcriptome / genetics
IF 3.998
リソース情報
ヒト・動物細胞 KGN(RCB1154)