RRC ID 72496
Author Hashizume K, Hatanaka Y, Fukuda I, Sano T, Yamaguchi Y, Tani Y, Danno G, Suzuki K, Ashida H.
Title N-acetyl-L-cysteine suppresses constitutive expression of CD11a/LFA-1alpha protein in myeloid lineage.
Journal Leuk Res
Abstract We investigated the possible involvement of redox regulation in constitutive expression of CD11a/LFA-1alpha, a leukocyte integrin alpha subunit, in myeloid cells using antioxidants. In unstimulated HL-60 cells, CD11a/LFA-1alpha was highly expressed, however, no expression of CD11b and CD11c proteins was detected. N-acetyl-L-cysteine (NAC) markedly down-regulated CD11a/LFA-1alpha expression in a dose-dependent manner. The down-regulated CD11a/LFA-1alpha expression was gradually recovered when NAC was deprived 24h after treatment. Pyrrolidine dithiocarbamate (PDTC) also suppressed the level of expression CD11a/LFA-1alpha protein, although the effect of PDTC was less potent than NAC. Both NAC and PDTC suppressed NF-kappaB binding activity to consensus DNA probe, and this result was correlated with a suppressive effect to CD11a/LFA-1alpha expression. Furthermore, NAC also down-regulated CD11a/LFA-1alpha expression in both U937 cells and peripheral blood monocytes. These results indicated that the constitutive CD11a/LFA-1alpha expression in the myeloid lineage is implicated in oxidative stress occurring spontaneously, suggesting that alteration of the intracellular redox state using antioxidants may be effective in the modulation of cell adhesion associated with extravasation in leukocytes, at least, in myeloid cells.
Volume 26(10)
Pages 939-44
Published 2002-10-1
DOI 10.1016/s0145-2126(02)00037-1
PII S0145212602000371
PMID 12163056
MeSH Acetylcysteine / pharmacology* Antioxidants / pharmacology Cell Lineage DNA Probes / metabolism Down-Regulation / drug effects HL-60 Cells Humans Lymphocyte Function-Associated Antigen-1 / drug effects Lymphocyte Function-Associated Antigen-1 / metabolism* Monocytes / drug effects Monocytes / metabolism Myeloid Cells / drug effects* Myeloid Cells / metabolism NF-kappa B / metabolism Oxidation-Reduction Oxidative Stress Protein Binding / drug effects Pyrrolidines / pharmacology Reactive Oxygen Species / pharmacology Thiocarbamates / pharmacology U937 Cells
IF 2.214
Human and Animal Cells U-937 DE-4(RCB0435)