論文 - 詳細
| RRC ID | 72505 |
|---|---|
| 著者 | Morishita A, Kumabe S, Nakatsuka M, Iwai Y. |
| タイトル | A histological study of mineralised tissue formation around implants with 3D culture of HMS0014 cells in Cellmatrix Type I-A collagen gel scaffold in vitro. |
| ジャーナル | Okajimas Folia Anat Jpn |
| Abstract |
We cultured HMS0014 Yub621b cells within a 3D collagen gel scaffold (Cellmatrix Type I-A) and aimed to study the fate and contribution of human bone-derived mesenchymal stem cells (MSCs) in the guided bone regeneration(GBR)-engineered tissue which has developed around the titanium (Ti) test dental implant (IP) in vitro. The light microscopy (LM) and transmission electron microscopy (TEM) results of the peri-IP tissue indicated that collagen fibrils of the Cellmatrix Type I-A gel were accumulated and fabricated to provide a 3D meshwork for proliferation and differentiation of the HMS0014 cells in the top (cell) layer; mineralisation of the GBR tissue had commenced since day 1 and became markedly deposited between days 7 and 14 of the experiment. TEM observation revealed sedimentation of cement line at the periphery of the interwoven Cellmatrix fibres and fibrils in the ECM scaffold of the GBR tissue; matrix vesicle-mediated and appositional collagen-mediated mineralisation were identified in the peri-IP ECM scaffold. The fine structure study of the plurimorphic osteoblast(Ob)-like osteogeneic cells demonstrated numerous membranous organelles related with vesicular trafficking, secretion and endocytosis in the cytoplasm; well-developed cytoskeleton networks and intercellular junctional complexes were also observed. The specimens on fluorescence immunohistochemistry (IHC) by confocal laser-scanning microscopy (LSM) showed the expression of LC3 and Cx43 associated with autophagic-lysosomal degeneration pathway and signal conduction mediated with gap junctions (GJS) in maintaining tissue homeostasis of the Ob-like cells which grew and degenerated in the 3D scaffold. Results from this in vitro study suggest that Ob-like HMS0014 cells actively regulate turnover of the peri-IP ECM to recapitulate the development and formation of osteoid tissue-engineered material which might contribute to augment osseointegration around the dental implant. |
| 巻・号 | 91(3) |
| ページ | 57-71 |
| 公開日 | 2014-1-1 |
| DOI | 10.2535/ofaj.91.57 |
| PMID | 25797459 |
| MeSH | Cell Differentiation / physiology Cell Proliferation / physiology Cells, Cultured Cellular Microenvironment / physiology Collagen Type I / physiology* Cytoskeleton / physiology Cytoskeleton / ultrastructure Dental Implants* Extracellular Matrix / physiology* Homeostasis / physiology Humans In Vitro Techniques Intercellular Junctions / physiology Intercellular Junctions / ultrastructure Mesenchymal Stem Cells / cytology* Mesenchymal Stem Cells / physiology* Mesenchymal Stem Cells / ultrastructure Microscopy, Confocal Microscopy, Electron, Transmission Osteogenesis / physiology* Time Factors Tissue Engineering / methods* Tissue Scaffolds* Titanium |
| リソース情報 | |
| ヒト・動物細胞 | Yub621b(HMS0014) |