論文 - 詳細
| RRC ID | 72513 |
|---|---|
| 著者 | Kondo Y, Yoshihashi S, Mimori K, Ogihara R, Kanehama Y, Maki Y, Enosawa S, Kurose K, Iwao T, Nakamura K, Matsunaga T. |
| タイトル | Selective culture method for hepatocyte-like cells differentiated from human induced pluripotent stem cells. |
| ジャーナル | Drug Metab Pharmacokinet |
| Abstract |
This study aimed to establish culture conditions which are able to give the differentiation of induced pluripotent (iPS) cells to hepatocytes. To this end, we examined the usefulness of a culture medium containing the components involved in the intermediary metabolism in the liver. More specifically, we examined the effect of the "modified L-15 medium" containing galactose, phenylalanine and ornitine, but deprived of glucose, tyrosine, arginine and pyruvic acid. The medium was altered according to changes in the expression of enzymes that participate in liver-specific pathways. After 25 days of differentiation, the differentiated cells expressed hepatocyte markers and drug-metabolizing enzymes. These expression levels were increased using modified L-15 medium. The survival of human fetal liver cells and the death of human fibroblasts were observed during culture in modified L-15 medium. Most of the cells that differentiated from human iPS cells using modified L-15 medium were stained by anti-human albumin antibody. These results suggest that iPS cells can be converted to high purity-differentiated hepatocytes by cultivating them in modified L-15 medium. |
| 巻・号 | 29(5) |
| ページ | 407-13 |
| 公開日 | 2014-1-1 |
| DOI | 10.2133/dmpk.dmpk-14-rg-022 |
| PII | DN/JST.JSTAGE/dmpk/DMPK-14-RG-022 |
| PMID | 24785642 |
| MeSH | Cell Culture Techniques* Cell Differentiation* / drug effects Cell Survival / drug effects Culture Media / chemistry Culture Media / pharmacology Hepatocytes / cytology* Hepatocytes / drug effects Hepatocytes / enzymology Hepatocytes / metabolism Humans Induced Pluripotent Stem Cells / cytology* Induced Pluripotent Stem Cells / drug effects |
| IF | 2.772 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | Hep G2(RCB1886) |