RRC ID 72610
Author Kubota SI, Takahashi K, Nishida J, Morishita Y, Ehata S, Tainaka K, Miyazono K, Ueda HR.
Title Whole-Body Profiling of Cancer Metastasis with Single-Cell Resolution.
Journal Cell Rep
Abstract Stochastic and proliferative events initiated from a single cell can disrupt homeostatic balance and lead to fatal disease processes such as cancer metastasis. To overcome metastasis, it is necessary to detect and quantify sparsely distributed metastatic cells throughout the body at early stages. Here, we demonstrate that clear, unobstructed brain/body imaging cocktails and computational analysis (CUBIC)-based cancer (CUBIC-cancer) analysis with a refractive index (RI)-optimized protocol enables comprehensive cancer cell profiling of the whole body and organs. We applied CUBIC-cancer analysis to 13 mouse models using nine cancer cell lines and spatiotemporal quantification of metastatic cancer progression at single-cell resolution. CUBIC-cancer analysis suggests that the epithelial-mesenchymal transition promotes not only extravasation but also cell survival at metastatic sites. CUBIC-cancer analysis is also applicable to pharmacotherapeutic profiling of anti-tumor drugs. CUBIC-cancer analysis is compatible with in vivo bioluminescence imaging and 2D histology. We suggest that a scalable analytical pipeline with these three modalities may contribute to addressing currently incurable metastatic diseases.
Volume 20(1)
Pages 236-250
Published 2017-7-5
DOI 10.1016/j.celrep.2017.06.010
PII S2211-1247(17)30806-9
PMID 28683317
MeSH Animals Cell Line, Tumor Epithelial-Mesenchymal Transition Female Male Mice Mice, Inbred BALB C Mice, Inbred C57BL Neoplasm Metastasis Neoplasms, Experimental / diagnostic imaging* Neoplasms, Experimental / pathology Optical Imaging / methods* Single-Cell Analysis / methods* Tumor Microenvironment Whole Body Imaging / methods*
IF 8.109
DNA material CSII-CMV-RfA (RDB04386) CSII-EF-RfA (RDB04387) CS-CDF-CG-PRE (RDB04379) pCMV-VSV-G-RSV-Rev (RDB04393) pCAG-HIVgp (RDB04394)