論文 - 詳細
| RRC ID | 72652 |
|---|---|
| 著者 | Tatsuno T, Ishigaki Y. |
| タイトル | C-terminal short arginine/serine repeat sequence-dependent regulation of Y14 (RBM8A) localization. |
| ジャーナル | Sci Rep |
| Abstract |
Y14 (RBM8A) is an RNA recognition motif-containing protein that forms heterodimers with MAGOH and serves as a core factor of the RNA surveillance machinery for the exon junction complex (EJC). The role of the Y14 C-terminal serine/arginine (RS) repeat-containing region, which has been reported to undergo modifications such as phosphorylation and methylation, has not been sufficiently investigated. Thus, we aimed to explore the functional significance of the Y14 C-terminal region. Deletion or dephosphorylation mimic mutants of the C-terminal region showed a shift in localization from the nucleoplasmic region; in addition, the C-terminal RS repeat-containing sequence itself exhibited the potential for nucleolar localization. Additionally, the regulation of Y14 localization by the C-terminal region was further found to be exquisitely controlled by MAGOH binding. Cumulatively, our findings, which demonstrated that Y14 localization is regulated not only by the previously reported N-terminal localization signal but also by the C-terminal RS repeat-containing region through phosphorylation and MAGOH binding to Y14, provide new insights for the mechanism of localization of short RS repeat-containing proteins. |
| 巻・号 | 8(1) |
| ページ | 612 |
| 公開日 | 2018-1-12 |
| DOI | 10.1038/s41598-017-18765-1 |
| PII | 10.1038/s41598-017-18765-1 |
| PMID | 29330450 |
| PMC | PMC5766523 |
| MeSH | Arginine / metabolism Binding Sites Cell Nucleolus / metabolism HeLa Cells Humans Intranuclear Space / metabolism* Nuclear Proteins / metabolism* Phosphorylation Protein Binding Protein Domains Protein Transport RNA-Binding Proteins / chemistry* RNA-Binding Proteins / genetics RNA-Binding Proteins / metabolism* Serine / metabolism |
| IF | 3.998 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HeLa(RCB0007) |