RRC ID 72652
Author Tatsuno T, Ishigaki Y.
Title C-terminal short arginine/serine repeat sequence-dependent regulation of Y14 (RBM8A) localization.
Journal Sci Rep
Abstract Y14 (RBM8A) is an RNA recognition motif-containing protein that forms heterodimers with MAGOH and serves as a core factor of the RNA surveillance machinery for the exon junction complex (EJC). The role of the Y14 C-terminal serine/arginine (RS) repeat-containing region, which has been reported to undergo modifications such as phosphorylation and methylation, has not been sufficiently investigated. Thus, we aimed to explore the functional significance of the Y14 C-terminal region. Deletion or dephosphorylation mimic mutants of the C-terminal region showed a shift in localization from the nucleoplasmic region; in addition, the C-terminal RS repeat-containing sequence itself exhibited the potential for nucleolar localization. Additionally, the regulation of Y14 localization by the C-terminal region was further found to be exquisitely controlled by MAGOH binding. Cumulatively, our findings, which demonstrated that Y14 localization is regulated not only by the previously reported N-terminal localization signal but also by the C-terminal RS repeat-containing region through phosphorylation and MAGOH binding to Y14, provide new insights for the mechanism of localization of short RS repeat-containing proteins.
Volume 8(1)
Pages 612
Published 2018-1-12
DOI 10.1038/s41598-017-18765-1
PII 10.1038/s41598-017-18765-1
PMID 29330450
PMC PMC5766523
MeSH Arginine / metabolism Binding Sites Cell Nucleolus / metabolism HeLa Cells Humans Intranuclear Space / metabolism* Nuclear Proteins / metabolism* Phosphorylation Protein Binding Protein Domains Protein Transport RNA-Binding Proteins / chemistry* RNA-Binding Proteins / genetics RNA-Binding Proteins / metabolism* Serine / metabolism
IF 3.998
Human and Animal Cells HeLa(RCB0007)