RRC ID 72657
Author Zhao T, Xiao Y, Huang B, Ran MJ, Duan X, Wang YF, Lu Y, Yu XQ.
Title A dual role of lola in Drosophila ovary development: regulating stem cell niche establishment and repressing apoptosis.
Journal Cell Death Dis
Abstract In Drosophila ovary, niche is composed of somatic cells, including terminal filament cells (TFCs), cap cells (CCs) and escort cells (ECs), which provide extrinsic signals to maintain stem cell renewal or initiate cell differentiation. Niche establishment begins in larval stages when terminal filaments (TFs) are formed, but the underlying mechanism for the development of TFs remains largely unknown. Here we report that transcription factor longitudinals lacking (Lola) is essential for ovary morphogenesis. We showed that Lola protein was expressed abundantly in TFCs and CCs, although also in other cells, and lola was required for the establishment of niche during larval stage. Importantly, we found that knockdown expression of lola induced apoptosis in adult ovary, and that lola affected adult ovary morphogenesis by suppressing expression of Regulator of cullins 1b (Roc1b), an apoptosis-related gene that regulates caspase activation during spermatogenesis. These findings significantly expand our understanding of the mechanisms controlling niche establishment and adult oogenesis in Drosophila.
Volume 13(9)
Pages 756
Published 2022-9-2
DOI 10.1038/s41419-022-05195-9
PII 10.1038/s41419-022-05195-9
PMID 36056003
PMC PMC9440207
MeSH Animals Apoptosis / genetics Cell Differentiation / genetics Drosophila* / metabolism Drosophila Proteins* / genetics Drosophila Proteins* / metabolism Drosophila melanogaster / metabolism Female Male Ovary / metabolism Stem Cell Niche / genetics Transcription Factors / genetics Transcription Factors / metabolism
IF 6.304
Drosophila 12052R-1