RRC ID 72837
Author Nagai K, Tahara-Hanaoka S, Morishima Y, Tokunaga T, Imoto Y, Noguchi E, Kanemaru K, Imai M, Shibayama S, Hizawa N, Fujieda S, Yamagata K, Shibuya A.
Title Expression and function of Allergin-1 on human primary mast cells.
Journal PLoS One
Abstract Mast cells (MC) play an important role in allergic and non-allergic immune responses. Activation of human MC is modulated by several cell surface inhibitory receptors, including recently identified Allergin-1 expressed on both human and mouse MC. Although Allergin-1 suppresses IgE-mediated, mast cell-dependent anaphylaxis in mice, the expression profile and function of Allergin-1 on human primary MC remains undetermined. Here, we established a seven-color flow cytometry method for assessing expression and function of a very small number of human primary MC. We show that Allergin-1S1, a splicing isoform of Allergin-1, is predominantly expressed on human primary MC in both bronchoalveolar lavage (BAL) fluid and nasal scratching specimens. Moreover, Allergin-1S1 inhibits IgE-mediated activation from human primary MC in BAL fluid. These results indicate that Allergin-1 on human primary MC exhibits similar characteristics as mouse Allergin-1 in the expression profile and function.
Volume 8(10)
Pages e76160
Published 2013-1-1
DOI 10.1371/journal.pone.0076160
PII PONE-D-13-27350
PMID 24116093
PMC PMC3792105
MeSH Bronchoalveolar Lavage Fluid / cytology Flow Cytometry Humans Immunoglobulin E / immunology Immunoglobulin E / metabolism Mast Cells / cytology Mast Cells / immunology Mast Cells / metabolism* Receptors, Immunologic / genetics Receptors, Immunologic / metabolism*
IF 2.74
Cord blood stem cells for research