| RRC ID |
72838
|
| 著者 |
Nakamori Y, Liu B, Ohishi K, Suzuki K, Ino K, Matsumoto T, Masuya M, Nishikawa H, Shiku H, Hamada H, Katayama N.
|
| タイトル |
Human bone marrow stromal cells simultaneously support B and T/NK lineage development from human haematopoietic progenitors: a principal role for flt3 ligand in lymphopoiesis.
|
| ジャーナル |
Br J Haematol
|
| Abstract |
The regulation of human early lymphopoiesis remains unclear. B- and T-lineage cells cannot develop simultaneously with conventional stromal cultures. Here we show that telomerized human bone marrow stromal cells supported simultaneous generation of CD19(+) CD34(lo/-) CD10(+) cyCD79a(+) CD20(+/-) VpreB(-) pro-B cells and CD7(+) CD34(+) CD45RA(+) CD56(-) cyCD3(-) early T/Natural Killer (NK) cell precursors from human haematopoietic progenitors, and the generation of both lymphoid precursors was promoted by flt3 ligand (flt3L). On the other hand, stem cell factor or thrombopoietin had little or no effect when used alone. However, both acted synergistically with flt3L to augment the generation of both lymphoid precursors. Characteristics of these lymphoid precursors were evaluated by gene expression profiles, rearrangements of IgH genes, or replating assays. Similar findings were observed with primary human bone marrow stromal cells. Notably, these two lymphoid-lineage precursors were generated without direct contact with stromal cells, indicating that early B and T/NK development can occur, at least in part, by stromal cell-derived humoral factors. In serum-free cultures, flt3L elicited similar effects and appeared particularly important for B cell development. The findings of this study identified the potential of human bone marrow stromal cells to support human early B and T lymphopoiesis and a principal role for flt3L during early lymphopoiesis.
|
| 巻・号 |
157(6)
|
| ページ |
674-86
|
| 公開日 |
2012-6-1
|
| DOI |
10.1111/j.1365-2141.2012.09109.x
|
| PMID |
22463758
|
| MeSH |
Adjuvants, Immunologic / pharmacology*
Antigens, CD / biosynthesis
B-Lymphocytes / cytology
B-Lymphocytes / metabolism*
Cell Communication / drug effects
Cell Communication / physiology
Cells, Cultured
Female
Gene Expression Regulation / drug effects
Gene Expression Regulation / physiology
Gene Rearrangement, B-Lymphocyte, Heavy Chain / drug effects
Gene Rearrangement, B-Lymphocyte, Heavy Chain / physiology
Humans
Killer Cells, Natural / cytology
Killer Cells, Natural / metabolism*
Lymphoid Progenitor Cells / cytology
Lymphoid Progenitor Cells / metabolism*
Lymphopoiesis / drug effects*
Lymphopoiesis / physiology
Male
Membrane Proteins / pharmacology*
Stromal Cells / cytology
Stromal Cells / metabolism
T-Lymphocytes / cytology
T-Lymphocytes / metabolism*
|
| IF |
5.518
|
| リソース情報 |
| ヒト・動物細胞 |
StromaNKtert(RCB2350) |
