Reference - Detail
| RRC ID | 72839 |
|---|---|
| Author | Satoh J, Yamamura T, Arima K. |
| Title | The 14-3-3 protein epsilon isoform expressed in reactive astrocytes in demyelinating lesions of multiple sclerosis binds to vimentin and glial fibrillary acidic protein in cultured human astrocytes. |
| Journal | Am J Pathol |
| Abstract |
The 14-3-3 protein family consists of acidic 30-kd proteins expressed at high levels in neurons of the central nervous system. Seven isoforms form a dimeric complex that acts as a molecular chaperone that interacts with key signaling components. Recent studies indicated that the 14-3-3 protein identified in the cerebrospinal fluid of various neurological diseases including multiple sclerosis (MS) is a marker for extensive brain destruction. However, it remains unknown whether the 14-3-3 protein plays an active role in the pathological process of MS. To investigate the differential expression of seven 14-3-3 isoforms in MS lesions, brain tissues of four progressive cases were immunolabeled with a panel of isoform-specific antibodies. Reactive astrocytes in chronic demyelinating lesions intensely expressed beta, epsilon, zeta, eta, and sigma isoforms, among which the epsilon isoform is a highly specific marker for reactive astrocytes. Furthermore, protein overlay, mass spectrometry, immunoprecipitation, and double-immunolabeling analysis showed that the 14-3-3 protein interacts with both vimentin and glial fibrillary acidic protein in cultured human astrocytes. These results suggest that the 14-3-3 protein plays an organizing role in the intermediate filament network in reactive astrocytes at the site of demyelinating lesions in MS. |
| Volume | 165(2) |
| Pages | 577-92 |
| Published | 2004-8-1 |
| DOI | 10.1016/s0002-9440(10)63322-6 |
| PII | S0002-9440(10)63322-6 |
| PMID | 15277231 |
| PMC | PMC1618573 |
| MeSH | 14-3-3 Proteins Adult Aged Aged, 80 and over Animals Animals, Newborn Astrocytes / metabolism* Astrocytes / pathology Autopsy Cells, Cultured Demyelinating Diseases / metabolism* Demyelinating Diseases / pathology Female Fetus / pathology Glial Fibrillary Acidic Protein / metabolism* Humans Immunoblotting Male Mass Spectrometry Mice Middle Aged Multiple Sclerosis / metabolism* Multiple Sclerosis / pathology Precipitin Tests Protein Isoforms / metabolism Tyrosine 3-Monooxygenase / metabolism* Vimentin / metabolism* |
| IF | 3.491 |
| Altmetric score |
オルトメトリクス指標項目
|
| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 16 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | HeLa(RCB0007) |