RRC ID 72857
著者 Oizumi Y, Kaji T, Tashiro S, Takeshita Y, Date Y, Kanoh J.
タイトル Complete sequences of Schizosaccharomyces pombe subtelomeres reveal multiple patterns of genome variation.
ジャーナル Nat Commun
Abstract Genome sequences have been determined for many model organisms; however, repetitive regions such as centromeres, telomeres, and subtelomeres have not yet been sequenced completely. Here, we report the complete sequences of subtelomeric homologous (SH) regions of the fission yeast Schizosaccharomyces pombe. We overcame technical difficulties to obtain subtelomeric repetitive sequences by constructing strains that possess single SH regions of a standard laboratory strain. In addition, some natural isolates of S. pombe were analyzed using previous sequencing data. Whole sequences of SH regions revealed that each SH region consists of two distinct parts with mosaics of multiple common segments or blocks showing high variation among subtelomeres and strains. Subtelomere regions show relatively high frequency of nucleotide variations among strains compared with the other chromosomal regions. Furthermore, we identified subtelomeric RecQ-type helicase genes, tlh3 and tlh4, which add to the already known tlh1 and tlh2, and found that the tlh1-4 genes show high sequence variation with missense mutations, insertions, and deletions but no severe effects on their RNA expression. Our results indicate that SH sequences are highly polymorphic and hot spots for genome variation. These features of subtelomeres may have contributed to genome diversity and, conversely, various diseases.
巻・号 12(1)
ページ 611
公開日 2021-1-27
DOI 10.1038/s41467-020-20595-1
PII 10.1038/s41467-020-20595-1
PMID 33504776
PMC PMC7840980
MeSH Base Sequence Genetic Variation* Genome, Fungal* INDEL Mutation / genetics Mosaicism Multigene Family Nucleotides / genetics Phylogeny RNA, Fungal / genetics RecQ Helicases / genetics Schizosaccharomyces / genetics* Schizosaccharomyces / isolation & purification Telomere / genetics*
IF 12.121
リソース情報
酵母 FY18675 (972)